Curriculum Vitae

Garshasb Rigi, Ph.D

Associate Professor  of Molecular Genetics,

Department of Genetics,

Collage of Basic Science,

Shahrekord University.

Email: garshasbbiotech@gmail.com, garshasbiotech@sku.ac.ir

Education

Sep 2011- May 2014

National Institute of Genetic Engineering and Biotechnology

PhD student, Molecular Genetics

Tehran, Iran

Aug 2011– May 2012

Tarbiat Modares University · Department of Genetics (Faculty of Biological Sciences)

PhD student, Molecular Genetics

Iran · Tehrān

Oct 2009 – Sep 2011

Razi Vaccine and Serum Research Institute and Tehran PNU

MSc, Biotechnology and Molecular Genetics

Karaj, Iran

Aug 1996 – Feb 2000

Isfahan University of Technology

Bachelor of Science,

Eşfahān, Iran

Aug 1992 – Feb 1996

Excellent High school

Diploma, The Experimental Sciences

Borūjen, Chahār Maḩāll va Bakhtīārī, Iran

Teaching Experience

    Jan 2018–present

Environmental Biotechnology (Msc)

Shahrekord University

 

    Jan 2018–present

Virology

Shahrekord University

 

    Jan 2018–present

Biosafety

Shahrekord University

 

    Jan 2018–present

General Biochemistry

Shahrekord University

 

    Dec 2017–present

General Microbiology

Shahrekord University

 

    Dec 2017–present

Biochemistry 2

Shahrekord University

 

    Feb 2017–present

Immunology

Behbahan  Khatam Alanbia University of Technology

 

    Feb 2016–present

Forest Genetics

Behbahan  Khatam Alanbia University of Technology

 

    Feb 2013–present

Microbiology

Behbahan  Khatam Alanbia University of Technology

 

Dec 2011 – present

Virology

Behbahan  Khatam Alanbia University of Technology

Dec 2011 – present

Biochemistry

Behbahan  Khatam Alanbia University of Technology

Dec 2011 – present

Molecular and Cellular Biology

Behbahan  Khatam Alanbia University of Technology

Dec 2011 – present

Genetics

Behbahan  Khatam Alanbia University of Technology

Dec 2009 - 2011

Agricultural courses

 PNU of Boroujen

Research Experience

 2018

Jun 2014

Evaluation of Antibacterial, Antifungal, and Antioxidant Properties of  Narcissus Bulbs from Behbahan

Virtual screening for finding potential drug like chemicals which blocks extracellular domain of CD 47 as an anti-cancer target

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology

Behbahan, Iran

Finding a chemical inhibitor which prevents Ebola virus entrance

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology

Behbahan, Iran

The effect of compost on some photosynthetic parameters in three growth stages of lentil (Lens culinaris Medik) under drought stress.

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology

Behbahan, Iran

Effect of some factors on embryogenesis and regeneration in maize (Zea mays L.) anther culture

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology

Behbahan, Iran

2015

Estimating the amount of secreted recombinant protein A using Fussy Regression insight into; determination of the optimal production conditions

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology, Behbahan, Iran

2015

Listeriolysin inhibition and drug design

Vira Vigene research and technology institute,

2015

Molecular docking based screening of predicted potential inhibitors for VP40 from Ebola virus

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology, Behbahan, Iran

2015

In-silico inhibition of Sortase from Listeria monocytogenes as a drug design tool

Vira Vigene research and technology institute,

2015

Finding a new compound inhibitor of amyloid-beta protein with a drug model in order to inhibit the Alzheimer's disease

Vira Vigene research and technology institute,

Jun 2015

In-silico inhibition of Sortase from Listeria monocytogenes as a drug design tool

Vira Vigene research and technology institute,

Jun 2014

Virtual screening following molecular dynamics simulation for finding potential

inhibitors of enterotoxin B from Staphylococcus aureus

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology

Behbahan, Iran

Aug 2012 – May 2013

PhD Student

Tarbiat Modares University, Department of Genetics (Faculty of Biological Sciences)

Tehrān, Iran

Mar 2012 – present

Surface display of protein A on spore and vegetative cells of Bacillus subtilis, E. coli and chitosan later use as a biotechnological product

National Institute of Genetic Engineering and Biotechnology, Department of Industrial and Environmental Biotechnology

Tehrān, Iran

Feb 2011 – present

High level recombinant protein A production and detection of its effects on immune system

National Institute of Genetic Engineering and Biotechnology, Department of Molecular Genetics

Tehrān, Iran

Aug 2010 – present

Recombinant vaccine production project against Hyalomma anatolicum anatolicom

Razi Vaccine and Serum Research Institute, Department of Biotechnology

Karaj, Iran

Jul 2009

Recombinant influenza vaccine production project

Razi Vaccine and Serum Research Institute, Department of Biotechnology

Karaj, Iran

2011

The effect of Iranian medicine and lactobacillus supernatant on inhibition of Staphylococcus aureus sortase as a potential drug target (Seminar)

National Institute of Genetic Engineering and Biotechnology, Department of Molecular Genetics

Tehrān, Iran

2011

Protein A : Generation and Applications (Seminar)

National Institute of Genetic Engineering and Biotechnology, Department of Molecular Genetics

Tehrān, Iran

2011

Assisting in the research project of Sortase in vitro analysis and inhibition as a potential drug target Iranian medicine and lactobacillus supernatant

National Institute of Genetic Engineering and Biotechnology, Department of Molecular Genetics

Tehrān, Iran

2011

Assisting in the national research project of Invitro and invivo evaluation of antibacterial potential of Lactobacillus plantarum HKN 01 isolated from Iranian traditional dairy products

National Institute of Genetic Engineering and Biotechnology, Department of Molecular Genetics

Tehrān, Iran

2011

Assisting in the research project of Gene Isolation, Cloning, expression and characterization of a novel chitinase gene of Streptomyces sp.BJ03 (isolated from Caspian sea); An insight into its role in biological control

National Institute of Genetic Engineering and Biotechnology, Department of Molecular Genetics

Tehrān, Iran

2010

Assisting in administrating the project of investigating the effect of dearth on 12 varieties of Bermuda grass

Boroujen municipality, under supervision of Dr.Khorshid Razmjoo, lecturer of Isfahan University of Technology

2010

Administrating the study of investigating the effect of dearth on 10 varieties of Zoshia grass

Boroujen municipality, under supervision of Dr.Khorshid Razmjoo, lecturer of Isfahan University of Technology

2010

Administrating the study of investigating the effect of saltiness on 12 varieties of Bermuda grass

Boroujen municipality, under supervision of Dr.Khorshid Razmjoo, lecturer of Isfahan University of Technology

2010

Administrating the study of investigating the effect of saltiness on 10 varieties of Zoshia grass

Boroujen municipality, under supervision of Dr.Khorshid Razmjoo, lecturer of Isfahan University of Technology

2010

In vitro Tissue culture of Fritillaria imperialis (Seminar)

Tehran PNU, under supervision of Dr.Mohammad jafaraghayi, assistance professor of Seed and Plant Improvement Institute.

2010

Administrating the study of investigating the effect of saltiness on 12 varieties of Bermuda grass

Boroujen municipality, under supervision of Dr.Khorshid Razmjoo, lecturer of Isfahan University of Technology

2009

Acting as an assistant in the research project of the effect of aerating on the rice root in Hydroponic system

Under the supervision of Dr.Aghafakhr Mirlohi , the professor of Isfahan University of Technology

2009

Assisting in the national research project of improving the quality & quantity of Triticum turgidum l grain

Under the supervision of Dr. Ahmad arzani, the professor of Isfahan University of Technology.

2009

Cooperating in the research project of the outcomes of drought in Chaharmahal o Bakhtiari province in order to suggest the drought and salt resistant plant species

Shahrekord center of water – related studies

2009

Preparing the optimum utilizing plan of Siasard forest park

Boroujen municipality

2009

Preparing the optimum utilizing plan of Ahmadabad forest park

Boroujen municipality

2009

Preparing the complete plan of Boroujen area under the title of: “Green area the breathing lungs of the city “

Boroujen municipality

2009

Preparing the well organizing plan of Boroujen green area

Boroujen municipality

2009

Preparing the identity papers of Boroujen green area

Boroujen municipality

2009

Preparing the green house plan (designing, administration, care and economic justifications)

Boroujen municipality

2009

Preparing the women park plan of Boroujen

Boroujen municipality

2009

Preparation of the proposal of the isolation project of the green area water from drinkable water in Boroujen and management and supervising on preparing and administrating of the mentioned project after getting it approved

Boroujen municipality

2009

Suggesting the proposal for the wastes management, preparation, supervision and administrating after its approval

Boroujen municipality

 

Awards & Grants

Des 2015

Winning the title of the Outstanding Researcher in Behbahan Khatam AlanbiaUniversity of Technology

Des 2015

Winning the title of the Outstanding Researcher in College of Science of Behbahan Khatam AlanbiaUniversity of Technology

Des 2015

Winning the title of the Outstanding Researcher in Basidje of Behbahan Khatam AlanbiaUniversity of Technology

Sep 2015

Awarding the International England TUV academy certificate

Aug 2015

Winning the International Australian Academy PCMS award in the third congress of environment, energy and bio-defense

Aug 2014

Winning the title of the Outstanding and Excellence student in Ph. D Research

Aug 2010

Award: Being respected and gloried in the festival of science and technology endeavours, organization of scientific endeavourer.

Aug 2010

Award: Gaining the title of the distinguished elite in the festival manifestation of scientific self – belief and innovation

Jul 2009

Award: Winning the title of the distinguished student in MA course

Jun 2009

Award: Membership in the national foundation of the elite.

Jun 2009

Scholarship: Full educational scholarship of ministry of sciences , researches & technology

Skills & Activities

Skills

Genetic Analysis, Gel Electrophoresis, LC-MS, MOLDI-TOF MS, Protein Analysis, Chromatographic Techniques, Immunoassay Development, Protein Characterization, Promoter Analysis, Flow Cytometer, Bacterial Genetics, Molecular Genomics, Immunogenetics, Classical Genetics, Genetic Analysis, Analytical HPLC, Bradford Assay, SDS-PAGE, CTAB DNA extraction, Cell Culture, Cloning, DNA Amplification, ELISA, PCR, Western Blot, Dialysis, DNA, Electrophoresis, Enzymes, Immunoassay, Mass Spectrometry, Protein Engineering, Gene Expression, Genomics, Bioinformatics and Computational Biology, Cell Culture Techniques, HPLC-MS, Spectrophotometer, Genomic PCR, Genomic DNA Isolation, Statistical Design, High-Performance Liquid Chromatography (HPLC), Microbiology, Industrial and Environmental Biotechnology, Scientific Writing,  Protein Chemistry, Transfection, RNA Isolation, RT-PCR, Cell Biology, Biochemistry,  

know lots of bioinformatic softwares and sites, Sites or online softwares such as:, Pymol, Mascot, EMBL, MATCH, PDB, PIR, Pfam, KEGG, GMO, SRS, Ensemble, BLOCKS, PRINTS, PubMed, Proteomics, Tigers, smart, Sage, GEO, Entrez, map Viewer, EPD, EPO, USPTO,  PROSITE,  NEB Cutter, swiss-prot, patch,  UNIPROT, REACTOME, INTERPRO, PRODOM, RefSeq, Phyre, CDD, SignalP, MegAlign, UCSC, CDART, Primerselect, Unigene, MapDraw, AGRICOLA, AGRIS, SurfIP, Patent Lens, PSORT, SWISS-2DPAGE database, FindMod, iProclass, Gene Indices, SNPdb, CAMBIA, TIGRFAMs, Gene Ontology (GO), NCBI, BLAST, mFold, Clustal w2, I-TASSER, TargetP, peptide property calculator, ExPaSy, msproduct, eTBLAST,

 SAFTWARES such as:, Vector.NTI Advance, CLONEWIN, MEGA, Bioedith,  SeqMan, SPSS, OLIGO, Chromas, Genetyx, Protean, GeneMan, EditSeq, SnapGene Viewer, Gene Runner,  Molegro Molecular Viewer, Design – Expert, SigmaPlot, MagTran, Xcalibor, Spdbv,

 

For other Skills, please refer to:

 https://www.researchgate.net/profile/Garshasb_Rigi3/

 http://www.  ir.linkedin.com/pub/garshasb-rigi-ph-d/48/365/ba2/

 http://garshasbbiotech.blogfa.com/

https://www.sku.ac.ir/en/DepartmentGroup/ProfessorForm.aspx?ID=2342

https://www.sku.ac.ir/~rigi#

https://scholar.google.com/citations?user=T6YVtr4AAAAJ&hl=en

ORCID ID: http://orcid.org/0000-0003-2450-912X

Scopus ID (Author ID): 55255171200

https://www.scopus.com/authid/detail.uri?authorId=55255171200 

Researcher ID :   L-9597-2016

Profile URL :   http://www.researcherid.com/rid/L-9597-2016

https://www.mendeley.com/profiles/garshasb-rigi/

publons.com/a/1568808/

Languages

English
French
Persian

Scientific

Memberships

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Some Social, Cultural and political activities and executive positions and responsibilities in various situations & times

Bio safety Society of Iran

Genetics Society of Iran

Biotechnology Society of Iran

Iran Society for Development of Landscape & Environment Conservation

The Eastern Mediterranean Health Genomics and Biotechnology Network (EMGEN)

Bioinformatics society of Iran

 Iranian Society for Immunology & Allergy

 

Education Deputy of Faculty of Science, Shahrekord University

Real Member of the Scientific Committee of Biotechnology at Research Institute of Biotechnology, Shahrekord University

Member of the Department of Industrial Biotechnology at Research Institute of Biotechnology, Shahrekord University

Member of the Consumer Pattern Reform Committee of the Strategic Management Board of Shahrekord University

Member of the Council of Nanotechnology Research Center of Shahrekord University

Scientific Staff  of  Genetics Department,  Shahrekord University

The head of the scientific – researching committee of agronomy & plant breeding group in agriculture faculty of Isfahan University of technology

Scientific Committee of Biology at  National Institute of Genetic Engineering and Biotechnology

Central Committee of Nanotechnology at  Behbahan Khatam Alanbia University of Technology

The head of Faculty of Science, University of Behbahan Khatam Alanbia University of Technology.

Executive advisor to the university president and member of the Presidential Advisory Council in University of Behbahan Khatam Alanbia University of Technology

Real member of the Council for Research and Technology, University of Behbahan Khatam Alanbia University of Technology.

Real member of Professional Council for cultural and social, University of Behbahan Khatam Alanbia University of Technology.

Advisor of Association of Biology, University of Behbahan Khatam Alanbia University of Technology.

Advisor of Shahed and Gallantry students in Faculty of Science, University of Behbahan Khatam Alanbia University of Technology.

Actual member of staff of martyrs and veterans, University of Behbahan Khatam Alanbia University of Technology.

Education and Research Deputy of Faculty of Science, University of Behbahan Khatam Alanbia University of Technology.

Member of the Monitoring Committee on issues, University of Behbahan Khatam Alanbia University of Technology.

The head of green area portion in Boroujen municipality.

The head of the planning committee for the anniversary of municipalities’ foundation, in Boroujen municipality.

Manager of the optimal use of energy project in Boroujen municipality and successful management especially during the year of utilization pattern reform.

The promotion assistant in the agriculture org of Chaharmahal o Bakhtiari province.

Supervising engineer of the wheat project in Boroujen County.

The head of the glorification and celebrating the Fajr ceremonies in Boroujen and winning the excellence level in 3 successive years.

The head of education & instruction in Imam Hosseyn Basidj as a distinguished member.

Assisting and cooperating in Shohada Basidj site council namely in Salehin project.

Member of religious group of Ansarilmahdi in Imam Hosseyn mosque – Boroujen and acting as the head of Islamic researches project between all.

The manager of the Rana ziba cultivating engineering company.

The expert of Golshan cooperative productive company in the fields of Glass house produced plants & flowers.

Producing a huge change & transforming in Boroujen green space and receiving several letters of appreciation.

Playing a great role & having a great effect in sowing trees in Boroujen Township and receiving several letters of appreciation from different governors & Bosses of Boroujen natural resource administration.

Gaining success in fighting against & controlling the calamities plagues and epidemic diseases in Boroujen Township & receiving several letters of appreciation from the headmaster of agriculture office of Boroujen Township.

Serving as the councilor & adviser of the governor of Boroujen Township in the fields of employment of agriculture graduators.

Membership in the center for the Basidji (The Islamic revolution semi - military and cultural special forces) elite of Chaharmahal o Bakhtiari province.

Chief - editor of Payam Magazine, a political, economic and cultural magazine.

Managing Director and Owner of Aabrah Magazine, a Cultural and Social magazine.

Secretary of Fajr Cultural Center of Islamic Revolution in Boroujen

The brother successor of university student basij at National Institute of Genetic Engineering and Biotechnology.

Membership of  central committee of Nanotechnology at Behbahan Khatam Alania University of Technology

Design, installation, equipping and preparation of the guidelines and GLP of Nanobiotechnology Laboratory in Behbahan Khatam Alanbia University of Technology

Membership of Youth Organization of Red Crescent society

Senior advisor to the Negin Ab Engineering Co. for evaluation of criteria and methods for bioremediation of environmental pollution in the Zayandehroud dam area

Scientific consultant to the Negin Khak Engineering co. in investigating the ability of microorganisms in Dorahan artificial recharge project area for bioremediation of its environmental pollution

Advisor to the Consulting Engineers co. in biodegradation studies of soil and water contamination of artificial recharge project in Khanmirza District

Referee

Preparative Biochemistry & Biotechnology (ISI Journal)

Iranian Journal of breast disease (Journal)

Organic and Medicinal Chemistry Letters (ISI Journal)

Inflammation  (ISI journal)

Gene Reports (ISI Journal, Elsevier)

​Biotechnic & Histochemistry (ISI Journal, Elsevier)

Eurasian Journal of Medicine (ISI journal)

Asian Pacific Journal of Cancer Prevention (APJCP) (ISI journal)

Photochemistry and Photobiology(ISI Journal, Wiley)

Journal of Cellular Biochemistry (ISI Journal, Wiley)

Journal of Applied Biotechnology Reports

Journal of Immunology and Serum Biology

Sky Journal of Journal of Medicine and Medical Sciences (SJMMS)

Journal of Advances in Biology

Journal of Paramedical Sciences

Journal of Cell & Tissue

Scientific Journal of Ilam University of Medical Sciences

Scientific Journal of Fasa University of Medical Sciences

Journal of Shahid Sadoughi University of Medical Sciences

International Journal of Diabetes in Developing Countries

The first Iranian festival – fair of invention – innovation (Conference)

04th National Bioinformatic Congress of  Iran (Conference)

The First National Congress of Biological and Natural Resources Sciences

The first international conference on new findings of Agriculture, Natural Resources and Environment

The First International Conference on Environmental Engineering

2nd. International Congress of Agricultural Sciences and Environment

2nd comprehensive National Congress of Novel Findings of Biology

Second National Conference on Application of Science and New Technologies in Agriculture, Natural Resources and Environment

Third National Conference on Sustainable Agriculture and Natural Resources

Third National Conference on Environment, Energy and biological defense

Advancement of Chemical Sciences (ACS) (Journal)

Photochemistry and Photobiology (ISI) (The American Society of Photobiology, Wiley Online Library)

The First Iranian Scientific Conference of Biology and Horticultural Sciences

The 1st International Environment and Natural Resources Conference

The First Iranian Congress of Develop and Promote of Agricultural Sciences and Natural Resources and Environment

The 1st International Environment and Natural Resources Conference

3rd. International Congress of Biology and Ecology

International conference in new research of Chemistry and Chemical Engineering

The 1st Conference on Advances and Challenges in Science, Engineering and Technology

The first national conference on passive defense in agriculture, natural resources and the environment with a view to sustainable development

The first National Congress of strategies to achieve sustainable development in the areas of Science and Technology

Second National Congress of biology and natural sciences

The Second Iranian Scientific Conference of Biology and Horticultural Sciences

The 2nd International Environment and Natural Resources Conference

The Fourth Congress of Sustainable Agriculture and Natural Resources

The Second International Conference on Environmental Engineering

The second international conference on new findings of Agriculture, Natural Resources and Environment

The third international conference on applied research in chemistry and chemical Engineering & biology

The first international conference on new findings of Agriculture, Natural Resources and Environment

International Conference on Modern Research Results in Sciences, Engineering & Technology

3rd. International Congress of Agricultural Sciences, Food and Environment

Scientific Secretary of Iranian Journal of Plant Physiology and Biochemistry

3rd. Conference of Future Environmental Crisis

3rd. National Congress of biology and natural sciences

The Fifth Congress of Sustainable Agriculture and Natural Resources

2nd. International Conference on new research findings of sciences

Second International & Fourteenth National Iranian Crop Science Congress

Fifth National Conference on Environment, Energy and biological defense

The second national conference on passive defense in agriculture, natural resources and the environment with a view to sustainable development

The first national conference on crisis management, safety, health, environment and sustainable development

Member of editorial board of  Journal of Bioinformatics and Proteomics Review

Member of editorial board of  Journal of Immunology and Serum Biology

Member of editorial board of  Journal of Pharmacy & Pharmaceutics

Editorial Member of Journal of Environment and Health Science

Editorial Member of Letters in Health & Biological Sciences

Editorial Member of Journal of Stem Cell and Regenerative Biology

Editorial Member of Journal of Marine Biology and Aquaculture

Editorial Member of  European Journal of Clinical and Biomedical Sciences

Editorial Member of International Journal of Genomics, Proteomics, Metabolomics & Bioinformatics

Associate Editor of Global Journal of Nanomedicine (GJN)

Associate Editor of Global Journal of Pharmacy & Pharmaceutical Sciences (GJPPS)

 Associate Editor of International Journal of Cell Science & Molecular Biology (IJCSMB)

Associate Editor of International Journal of Environmental Sciences & Natural Resources

Scientific

Appreciations

Being respected and gloried in the festival of science and technology             endeavourers of the Islamic revolution guards, organization of scientific

Being appreciate at Department of Molecular Genetics, National Institute of  Genetic Engineering and Biotechnology

Being appreciate at National Institute of  Genetic Engineering and Biotechnology in honor of outstanding performance and dedication and “Excellence” in the research projects and for innovative and high-quality research

Scientific

Recommendations

Gholamreza Bakhshi Khaniki

Professor,

Head of Agricultural Biotechnology department,

Tehran PNU

Aghafakhr Mirlohi

Professor,

Department of Agronomy and Pant Breeding,

Isfahan University of Technology

Khorshid Razmjoo

Associated Professor , 

Department of Agronomy and Pant Breeding,

Isfahan University of Technology

Ahmad Arzani

Professor,

Department of Agronomy and Pant Breeding,

Isfahan University of Technology

Mohammad Reza Khajehpour

Associated Professor , 

Department of Agronomy and Pant Breeding,

Isfahan University of Technology

Hossain Samadi

Assistance Professor,

Director of Water Recourses Research Center,

Shahrekord University

Gholamreza Ahmdian

Assistance Professor,

Department of Industrial and Environmental Biotechnology,

National Institute of Genetic Engineering and Biotechnology

Kambiz Akbari

Assistance Professor,

Director of Division of  Industrial and Environmental Biotechnology,

National Institute of Genetic Engineering and Biotechnology

Mohammad Ali Malboobi

Associated Professor , 

Department of Plant Biotechnology,

National Institute of Genetic Engineering and Biotechnology

Amir Mousavi

Associated Professor , 

Department of Plant Biotechnology,

National Institute of Genetic Engineering and Biotechnology

Ali Asghar Karkhaneh

Associated Professor , 

Department of Industrial and Environmental Biotechnology,

National Institute of Genetic Engineering and Biotechnology

Gholam Ali Kardar

Associated Professor , 

Immunology, Asthma & Allergy Research Institute (IAARI),

Children's Medical Center,

Tehran University of Medical Sciences,

 

Books

Real-Time PCR, under publishing

 

Introduction to FlowCytometry (Translation)

 

Principles and Commonly used laboratory methods of molecular and cellular biology and genetic engineering techniques

 

Purifying Challenging Proteins, Principles and Methods

 

Super Genes (under review)

 

Journal Publications

Garshasb Rigi Samira Ghaedmohammadi Gholamreza Ahmadian: A comprehensive review on staphylococcal protein A (SpA): Its production and applications. Biotechnology ans Applied Biochemistry. 66 (3):454-464 (2019)

Mostafa Akbariqomi, Reza Heidari, Soraya Saleh Gargari, Mir Davood Omrani, Garshasb Rigi, Nafiseh Sadat Sanikhani, Hamid Kooshki, Fatemeh Mahmoudian, Mohammad Ali Mazlomi, Gholamreza Tavoosidana1: Evaluation and statistical optimization of a method for methylated cell-free fetal DNA extraction from maternal plasma. Journal of Assisted Reproduction and Genetics. 36 (5): 1029-1038 (2019).

Raham Armand, Garshasb Rigi, Tahereh Bahrami: Fuzzy Hybrid Least-Squares Regression Approach to Estimating the amount of the Extra Cellular Recombinant Protein A from Escherichis coli BL21. Scientific Journal of Ilam University of Medical sciences, 2019

Nezam Armand, Raheleh Ahmadpour, Saeedreza Hosseinzadeh, Garshasb Rigi: The effect of compost on some photosynthetic parameters in three growth stages of lentil (Lens culinaris Medik) under drought stress. Journal of Plant Researches. 31(4): 916-926 (2019).

Raheleh Ahmadpour; Nezam Armand; Saeed Reza Hosseinzadeh; Garshasb Rigi: Review the use of compost fertilizer in soil and its influence on antioxidant activity, physiological and biochemical characteristics of lentil (Lens culinaris Medik) under water stress. Iranian journal of Pulses Research. 10 (1):141-154 (2019)

Parisa Ghahremanifard, Nasim Rezaeinezhad, Garshasb Rigi, Fatemeh Ramezani, Gholamreza Ahmadian: Designing a novel signal sequence for efficient secretion of Candida antarctica lipase B in E. coli: The molecular dynamic simulation, codon optimization and statistical analysis approach. International Journal of Biological Macromolecules. 119 : 291-305 (2018)

Neda Ebrahimzadeh, Hajar Vaseghi, Garshasb Rigi, Gholamreza Esmaeeli Djavid, Shahla Mohammad Ganji, Amirreza Farhoud, Majid Pornour: DRD2 Dopamine Receptor and IL-1β Gene Expression Changes Involved in Wound Healing in Type 2 Diabetic Patients with Foot Ulcer. Journal of Mazandaran University of Medical Sciences. 28 (162) :47-58 (2018)

Raham Armand, Garshasb Rigi, Reza Alizadeh: Removal of Green 6 Direct Dye from Aqueous Solutions Using Immobilized Laccase Enzyme on Zinc Ferrite Nanoparticle. Journal of Rafsanjan University of Medical Sciences. 16 (9): 857-868 (2018)

Hajar Vaseghi, Majid Pornour, Gholamreza Esmaeeli Djavid, Garshasb Rigi, Shahla Mohammad Ganji, Leila Novin: Association of the gene expression variation of tumor necrosis factor-α and expressions changes of dopamine receptor genes in progression of diabetic sever foot ulcer. Iranian Journal of Basic Medical Sciences. 20(11): 1219-1213 (2017).

Ibrahim Torktaz, Jafar Hemmat, Ali Asghar Karkhane, Garshasb Rigi, Amin Rostami, Jafar Khezri, Reza Behroozi: Molecular Engineering of the Geobacillus stearothermophilus α-Amylase and Cel5E from Chlostridium thermocellim; In Silico Approach. Iranian Journal of Biotechnology (IJB). 16(3): 185-192 (2018).

Garshasb Rigi, Mohsen Farhadpour, Alireza Ghassempour, Gholamreza Ahmadian, Hassan Y. Aboul-Enein : Per Aqueous Liquid Chromatography (PALC) as a Simple Method for Native Separation of Protein A. Chromatographia. 80(11): 1633-1639 (2017)

Simin Khodaei, Mehdi Mohammadi, Samira Ghaedmohammadi, Garshasb Rigi, Reza Zadmard, Gholamreza Ahmadian: Covalent immobilization of protein A on chitosan and aldehyde double-branched chitosan as biocompatible carriers for IgG purification. )under review).

Garshasb Rigi, Ibrahim Torktaz, Ahmad Ismaili, Abbas Mohammadi Azhar: A novel inhibitor of Staphylococcus aureus enterotoxin B, an in silico approach.)under review).

Sara Ghafari, Matin Komeilian, Mohaddese sadat Hashemi, Sareh Oushani, Garshasb Rigi, Behnam Rashidieh, Kamran Yarahmadi, Fatemeh Khoddam: Molecular docking based screening of Listeriolysin-O for improved inhibitors . Bioinformation 13(5): 160-163 (2017)

Abbas Shali, Garshasb Rigi, Majid Pornour, Gholamreza Ahmadian: Expression and secretion of Cyan Fluorescent Protein (CFP) using chitinase promoter of Bacillus pumilus SG2 in B. subtilis. Iranian Biomedical Journal (IBJ) 21(4): 240-248 (2017)

Garshasb Rigi, Mohammad Vala Ashdar Nakhaei, Hoda Eidipour, Arshia Najimi, Fahimeh Tajik, Niloufar Taher, Kamran Yarahmadi: Virtual screening following rational drug design based approach for introducing new anti-amyloid beta aggregation agent. Bioinformation 13(2): 31-36 (2017)

Garshasb Rigi, Raham Armand, Ibrahim Torktaz: Ligand screening approach to find potential inhibitors of GP1 from Ebola Virus. Journal of Paramedical Sciences (JPS) 8(2): 42-45 (2017)

Garshasb Rigi, Raham Armand, Tahereh Bahrami: Estimation of the Amount of Recombinant Protein A Secretion Using Fuzzy Regression. Iranian Journal of Health Sciences 4 (3), 35-44 (2016)

Behnam Rashidieh, Zohreh Madani, Mahtab Khoshnejad Azam, Saeedeh Khalesi Maklavani, Newsha Ramezani Akbari, Shaghayegh Tavakoli, Garshasb Rigi: Molecular docking based virtual screening of compounds for inhibiting sortase A in L. monocytogenes. Bioinformation 11 (11), 501-505 (2015)

Kambiz Morabbi Heravi, Garshasb Rigi, Maryam Rezaei Arjomand, Amin Rostami, Gholamreza Ahmadian: An alternative bacterial expression system using Bacillus pumilus SG2 chitinase promoter. Iranian Journal of Biotechnology 13 (4), 17-23 (2015)

Seyedeh Zahra Hosseini, Payam Pour Mohammadi, Garshsb Rigi: Effect of induction medium on embryogenesis of maize anther culture and increase of normal haploid plantlet regeneration with wathman filter paper. Iranian Journal of Plant Physiology 5 (4), 1505- 1512 (2015)

Garshasb Rigi, Parisa Beyranvand, Samira Ghaedmohammadi, Sara Heidarpanah, Kambiz Akbari Noghabi, Gholamreza Ahmadian: Comparison of the extracellular full-length and truncated recombinant protein A production in Escherichia coli BL21 (DE3). Journal of Paramedical Sciences (JPS) 6(3): 37-47 (2015)

Danya Abazari, Mehrad Moghtadaei, Ali Behvarmanesh, Bahareh Ghannadi, Monireh Aghaei, Mahboobeh Behruznia, Garshasb Rigi: Molecular docking based screening of predicted potential inhibitors for VP40 from Ebola virus. Bioinformation 11(5): 243-247 (2015)

Samira Ghaedmohammadi, Garshasb Rigi, Reza Zadmard, Ezio Ricca, Gholamreza Ahmadian: Immobilization of Bioactive Protein A from Staphylococcus aureus (SpA) on the Surface of Bacillus subtilis Spores. Molecular Biotechnology 2015 Apr; DOI: 10.1007/s12033-015-9868-z

Garshasb Rigi, Samira Ghaed Mohammadi, Maryam Rezaei Arjomand, Gholamreza Ahmadian, Kambiz Akbari Noghabi: Optimization of extracellular truncated Staphylococcal Protein A (SpA) expression in Escherichia coli BL21 (DE3). Biotechnology and Applied Biochemistry 2014 Mar; 61(2):217-25;

Majid Vahed, Ebrahim Motalebi, Garshasb Rigi, Kambiz Akbari Noghabi, Mohammad Reza Soudi, Mehdi Sadeghi, Gholamreza Ahmadian: Improving chitinolytic activity of Bacillus pumilus SG2 by random mutagenesis. Journal of Microbiology and Biotechnology 2013 Nov 28; 23(11):1519-28;

Farideh Zakeri, Majid Sadeghizadeh, Mohammad Reza Kardan, Hossein Shahbani Zahiri, Gholamreza Ahmadian, Fatemeh Masoumi, Hakimeh Sharafi, Garshasb Rigi, Hojatollah Vali, Kambiz Akbari Noghabi: Differential proteome analysis of a selected bacterial strain isolated from a high background radiation area in response to radium stress. Journal of proteomics 05/2012; 75(15):4820-32.

H Sharafi, L Alidost, H Abassi, G Rigi, H Shahbani Zahiri, G Ahmadian, H Vali, K Akbari Noghabi: Antibacterial activity of probiotic Lactobacillus plantarum HK01: Effect of divalent metal cations and food additives on production efficiency of antibacterial compounds. Preparative Biochemistry & Biotechnology 01/2012;

Garshasb Rigi, Gholam Ali Kardar, Kambiz Akbari Noghabi, Gholamreza Ahmadian: Detection of protein A effects on immune system. Iranian journal of Immunology; 05/2014; 11: 786-787

Garshasb Rigi, Morteza Esfahanian, Khosrow Aghayipur, Reza Behrouzi, Gholamreza Ahmadian: Cloning and Sequencing of HAO3 gene of Hyalomma anatolicum anatolicum in unsecretory shuttle vector pPICZB. Modern Genetics Journal; 05/2012

 

Patents

High level extracellular recombinant protein A production (filing)

Development of simple and economical method for purifying protein A (SpA) using hydrophilic interaction liquid chromatography technique (filing)

 

Conference Proceedings

Garshasb Rigi, Ibrahim Torktaz: Application of In Silico Approaches Development of New Drugs Against Infectious Disease. The 14th International Congress of Immunology and Allergy of Iran, Tehran, Iran; 04/2018

Garshasb Rigi, Gholamreza Ahmadian: Evaluation of the Situation and Function of Immunoglobulin Binding Domains from Full length and Truncated Forms of Protein A. The 14th International Congress of Immunology and Allergy of Iran, Tehran, Iran; 04/2018

Garshasb Rigi, Seyedeh Azadeh Moosavian: Influenza vaccine production in plants. The 3rd National Conference on Environment, Energy and biological defense, Tehran, Iran, 2015

Garshasb Rigi, Zenab eispareh, Ebtesam Saeidi, Hamideh Zamanpour: Bioinformatic genomics and proteomics study of nerve growth factor. The 3rd National Conference on Environment, Energy and biological defense, Tehran, Iran, 2015

Garshasb Rigi, Donya eskandari: Investigating the genes involved in degradation of environmental pollutants and biotechnology application. The 3rd National Conference on Environment, Energy and biological defense, Tehran, Iran, 2015

Sahar Ghasemi, Garshasb Rigi: pollen and anther culture. The First National Congress of Biological and Natural Science, Tehran, Iran, 2015

Beyranvand, Rigi, Ghaedmohammadi, Rezaee Arjomand, G. R. Ahmadian: Expression and purification of Xcm(I) endonucleases from xanthomonas campestris in E. coli using pET expression system. 18th National and 6th International Congress of Biology in Iran, Tehran. Iran; 06/2014

Garshasb Rigi, Gholam Ali Kardar, Kambiz Akbari Noghabi, Gholamreza Ahmadian: Detection of protein A effects on immune system. 12th International Congress of Immunology and Allergy of Iran, Tehran, Iran; 05/2014

Zeynab Azad, Elahe Kamalzadeh, Anis Nozari, Forough Bakhtiyari, Hamideh Heydari, Garshasb Rigi: Insilico study of Gag polyprotein: potential target for investigating on HIV-diagnosis and therapy. First International and 13th Iranian Genetics Congress, Tehran, Iran; 05/2014

Sahar Ghasemi, Farzaneh Yazdani, Mahnaz Vahidian, Nahid Boveyrati, Garshasb Rigi: Bioinformatic Analysis of Homo sapiens ArfGAP with FG repeats 1 (AGFG1). First International and 13th Iranian Genetics Congress, Tehran, Iran; 05/2014

Samira Ghaedmohammadi, Garshasb Rigi, Majid Vahed, Gholamreza Ahmadian: Cloning, expression and secretion of protein A from S.aureus in B.subtilis. 8th Iranian Biotechnology Congress, Tehran, Iran; 09/2013

Garshasb Rigi, Samira Ghaed Mohammadi, Maryam Rezaei Arjomand, Majid Vahed, Gholamreza Ahmadian, Kambiz Akbari Noghabi: Cloning and expression of truncated Staphylococcal Protein A (SpA) in E. coli. 8th Iranian Biotechnology Congress, Tehran, Iran; 06/2013

Reza Behroozi, Nesa Jafari, Kambiz Akbari noghabi, Gholamreza Ahmadian, Kosar Rahimzadeh, Garshasb Rigi, Esmaeil Frozan: Biological control of charcoal rot (Macrophomina phaseolina) using Streptomyces sp. BJ03 isolated from North of Iran. 12th Iranian Genetics Congress, Tehran, Iran; 05/2013

Samira Ghaedmohammadi, Garshasb Rigi, Majid Vahed, Gholamreza Ahmadian: Bacillus subtilis genome-wide screening to search sortase and its potential substrates. 1st Tabriz International Life Science Conference, Tabriz, Iran; 05/2013

Garshasb Rigi, Samira Ghaed Mohammadi, Maryam Rezaei Arjomand, Gholamreza Ahmadian, Kambiz Akbari Noghabi: Cloning and expression of Full - length form of Staphylococcal Protein A (SpA) in E. coli. 1st Tabriz International Life Science Conference, Tabriz, Iran; 05/2013

Mohammad Sadegh Koosha, Ali Mirjalili, Majid Tebianian, Garshasb Rigi, Gholamreza Ahmadian: Cloning and Sequencing of Mycobacterium tuberculosis ESAT-6 gene in pEGFP-N1 as an eukaryotic expressions vector. 12th Iranian Genetics Congress, Tehran,Iran; 05/2012

Fatemeh Rafieenia, Shamseddin Ahmadi, Jalal Rostamzadeh, Sabrieh Amini, Garshasb Rigi: The Role Of NMDA Receptors In Tolerance And Dependence To The Morphine In The Striatum And The Prefrontal Cortex Of Rat Brain. 12th Iranian Genetics Cogress, Tehran, Iran; 05/2012

Hadi. Zarei Mahmoodabady, Garshasb Rigi, M.Morshedy: Investigating the Effect of Industrial Tile and ceramic Wastewater on Groundwater Pollution (Case Study: Yazd Province). 2nd International Conference on Environmental Science and Technology, Singapore; 02/2011

Garshasb Rigi, H. Zarei, h Mahmoodabady, M. Chabok, Fa. Morady: Investigating the Potential of Recycling Urban Solid Wastes (Case Study: Ardakan City). The First International Conference on Sustainable Urbanization, Faculty of Construction and Land Use , Hong Kong Polytechnic University; 06/2010

Garshasb Rigi, H. Zarei Mahmoodabadi: Investigation of Wastewater Treatment Sludge, Yard Trimming Waste, and Food Waste Combination in Vermicompost Quality, a Case Study of Yazd, Iran. The First International Conference on Sustainable Urbanization, Faculty of Construction and Land Use, The Hong Kong Polytechnic University; 06/2010

Garshasb Rigi, Mashayekhpoor Saied, Aghaiepour Khosrow: Cloning and sequencig of the Influenza A (H9N2) M2e - HSP70 fusion protein in prokaryotic expression vector in order to recombinant vaccine production. 2nd International Veterinary Poultry Congress, Tehran, Iran; 02/2010

Garshasb Rigi, Aghayipor Khosrow, Mashayekhpour Saeid, Ebrahimi Seyed mahmoud: cloning & sequencing of HAO3 gene ( Hyalomma Anatolicum Anatolicum) in secretory shuttle vector pPICZαA. The First National Congress on of Veterinary Laboratory Science, Tehran, Iran; 10/2009

Genes identified, sequenced and submitted in the Gene Bank, National Center for Biotechnology Information (NCBI), ENA in Europe and the DNA Data Bank of Japan :

ACCESSION No: MH361761

Mycobacterium tuberculosis strain GR early secretory antigenic target 6 kDa protein (esxA) gene, complete cds.

              protein_id="AXL96670"

ACCESSION No: MH370480

Exiguobacterium sp. HA2 isocitrate dehydrogenase (citC) gene, partial cds.

protein_id="AXL96671"

ACCESSION No: MH378445

Exiguobacterium sp. HA2 DNA-directed RNA polymerase beta (rpoB) gene, partial cds.

protein_id="AXL96672"

ACCESSION No: MH378775

Exiguobacterium sp. HA2 class I heat shock protein (hsp70) gene, partial cds.

protein_id="AXL96673"

ACCESSION No: MH379139

Exiguobacterium sp. HA2 universal major cold shock protein (csp) gene, complete cds.

protein_id="AXL96674"

ACCESSION No: MH325952

Hyalomma anatolicum anatolicum HAO3 (HAO3) gene, complete cds

              protein_id="AXL96675"

ACCESSION No: MH379633

Narcissus tazetta L. cv. Shahla-e- Behbahan norbelladine 4'-O-methyltransferase (N4OMT4) mRNA,  complete cds.

              protein_id="AXL96676"

ACCESSION No: MH388024

Synthetic construct hemagglutinin (HA) gene, partial  cds.

              protein_id="AWW21439"

ACCESSION No: MH368127

Synthetic construct segment 4 hemagglutinin (HA) gene, complete cds.

              protein_id="AWL07886.1"

ACCESSION No: MH360730

Influenza A virus (A/chicken/Karaj/GHA/2017(H9N2)) segment 4 hemagglutinin (HA) gene, complete cds.              protein_id="AWK91180.1"

ACCESSION No: MH360730

Influenza A virus (A/chicken/Karaj/GM2/2017(H9N2)) segment 7 matrix protein 2 (M2) and matrix protein 1 (M1) genes, complete cds.

proteins_id="AWK24095.1", "AWK24094.1"

Conference Attending

          4th congress of agronomy & plant breeding in Iran – Karaj

          6th congress of agronomy & plant breeding in Iran – Mazandaran University, Babolsar

          7th congress of agronomy & plant breeding in Iran – Karaj

          5th national conference of waste and residue management in Mashhad

Workshop and Course Participating

          The green area management course at the 3rd national conference on green space and metropolitan sights at Kish Island and receiving the certificate from the organization of the state municipalities

          The watering management and drought crisis management in green area course in Isfahan

          The waste management course in Isfahan and receiving certificate from the state organization of municipalities and Isfahan waste org

          The waste management course in Shiraz and receiving certificate from the state organization of municipalities and Shiraz waste org

          Growing and production of decorating flowers course of the Education Complex of Agriculture org of Chaharmahal o Bakhtiari province

           Good Laboratory Practice (GLP) workshop, National Institute of Genetic Engineering and Biotechnology.

           Nanotechnology workshop, National Institute of Genetic Engineering and Biotechnology.

           Next Generation of Recombinant Drugs workshop in Specialized Conference on Job Opportunities and Research Fields on Drug Design.

          Next Generation of Biological Drugs workshop in Specialized Conference on Job Opportunities and Research Fields on Drug Design.

     Workshop Teaching

Genetic Engineering workshop instructor at Iranian Academic Center for Education Culture and Research (ACECR), Tehran University of Medical Science (TUMS) Branch

Recombinant Protein Production workshop instructor at Iranian Academic Center for Education Culture and Research (ACECR), Tehran University of Medical Science (TUMS) Branch

Gene Cloning and Molecular techniques workshop instructor at Iranian Academic Center for Education Culture and Research (ACECR), Tehran University of Medical Science (TUMS) Branch

Primer design and PCR workshop instructor at Iranian Academic Center for Education Culture and Research (ACECR), Tehran University of Medical Science (TUMS) Branch

Gene Expression  workshop instructor at Iranian Academic Center for Education Culture and Research (ACECR), Tehran University of Medical Science (TUMS) Branch

 

Bioinformatics (Genomics) workshop instructor at Behbahan Khatam Alanbia University of Technology.

Bioinformatics (Proteomics) workshop instructor at Behbahan Khatam Alanbia University of Technology.

Endnote software workshop instructor at Behbahan Khatam Alanbia University of Technology.

Design and analysis of microbiology experiments using Response Surface Methodology (RSM) and Design Expert and Sigma plat Softwares workshop instructor at Behbahan Khatam Alanbia University of Technology.

Stereotactic surgical techniques in laboratory animals, Western blotting basic techniques of molecular and cell culture techniques workshop instructor at 3rd. International Congress of Biology and Ecology

GLP workshop instructor at Behbahan Khatam Alanbia University of Technology.

Bioinformatics and Drug Design workshop in Specialized Conference on Job Opportunities and Research Fields on Drug Design.

Educational Projects and Income Opportunities in the Field of Cancer workshop in The First Festival and Symposium of Education and Employment in Iran’s Biotechnology.

Educational Projects and Income Opportunities in Gene/Cell therapy workshop in The First Festival and Symposium of Education and Employment in Iran’s Biotechnology.

Educational Projects and Income Opportunities in Biopharmaceutical workshop in The First Festival and Symposium of Education and Employment in Iran’s Biotechnology.

 

Teacher Evaluation Scores by students

 

Other notable scores

TOEFL ibt Score: 85/120

MSRT Score: 62/100

M.Sc. Thesis score: 19.25/20

Degree of M.Sc.  Thesis: Excellent

Ph.D. comprehensive exam score: 19.38/20

Ph.D. Thesis score: 19.95/20

Degree of Ph. D Thesis: Excellent

 

Persian cv

Image result for garshasb rigi

گرشاسب ریگی چشمه علی

دانشیار ژنتیک مولکولی

گروه ژنتیک، دانشکده علوم پایه، دانشگاه شهرکرد

ایمیل:garshasbiotech@sku.ac.ir 

          garshasbbiotech@gmail.com

 

سوابق تحصیلی:

دکتری ژنتیک مولکولی, پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری (دروس تئوری: دانشگاه تربیت مدرس)

کارشناسی ارشد مهندسی بیوتکنولوژی, دانشگاه پیام نور تهران مشترک با موسسه تحقیقات واکسن و سرم سازی رازی کرج

کارشناسی مهندسی زراعت و اصلاح نباتات, دانشگاه صنعتی اصفهان

 

سوابق اجرایی و فرهنگی:

  • معاون آموزشی دانشکده علوم پایه دانشگاه شهرکرد
  • عضو حقیقی کمیته تخصصی بیوتکنولوژی پژوهشکده زیست فناوری دانشگاه شهرکرد
  • عضو گروه پژوهشی بیوتکنولوژی صنعتی پژوهشکده زیست فناوری دانشگاه شهرکرد
  • عضو کمیته اصلاح الگوی مصرف شورای راهبردی مدیریت سبز دانشگاه شهرکرد
  • عضو شورای مرکز پژوهشی فناوری نانو دانشگاه شهرکرد
  • عضو هیات علمی گروه ژنتیک دانشگاه شهرکرد
  • ریاست دانشکده علوم پایه دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان
  • مشاور اجرایی رییس دانشگاه و عضو شورای مشورتی ریاست دانشگاه صنعتی خاتم الانبیاء(ص) بهبهان
  • عضو حقیقی شورای پژوهش و فناوری دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان
  • عضو حقیقی شورای تخصصی فرهنگی و اجتماعی دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان
  • استاد مشاور انجمن علمی زیست شناسی دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان
  • استاد مشاور دانشجویان شاهد و ایثارگر دانشکده علوم پایه دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان
  • معاون آموزشی و پژوهشی دانشکده علوم پایه دانشگاه صنعتی خاتم الانبیاء(ص) بهبهان.
  • عضو حقیقی ستاد شاهد و ایثارگر دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان
  • فعال فرهنگی.
  • عضو کمیته ناظر بر فعالیت نشریات دانشگاهی دانشگاه صنعتی خاتم الانبیاء(ص) بهبهان
  • عضو هیات علمی دانشگاه صنعتی خاتم الانبیاء(ص) بهبهان
  • مدیرمصرف بهینه انرژی شهرداری بروجن ومدیریت موفق بویژه در سال اصلاح الگوی مصرف.
  • مسوول کمیته فضاسازی شهربروجن دردهه مبارک فجر و کسب عنوان شهر برتر طی دوسال متوالی در استان. 
  • همکاری در نظارت بر برگزاری انتخابات شورای اسلامی شهر . 
  • مسوول آموزش پایگاه امام حسین (ع) بروجن به عنوان بسیجی فعال .
  • مشاور فرماندار شهرستان بروجن در زمینه اشتغال فارغ التحصیلان کشاورزی.
  • جانشین بسیج دانشجویی پژوهشگاه ملی مهندسی ژنتیک و بیوتکنولوژی
  • عضویت درشورای پایگاه بسیج شهداء بروجن از جمله به عنوان سرگروه حلقه یک طرح صالحین.
  • عضویت درهیئت مذهبی انصارالمهدی (عج) مسجدامام حسین (ع) بروجن و ازجمله فعالیت به عنوان مدیر پژوهشهای اسلامی.
  • همکاری با بنیادغدیردرشهرستان بروجن.
  • سر دبیر ماهنامه خبری تحلیلی پیام.
  • مدیرمسئول و صاحب امتیاز دوهفته نامه فرهنگی، اجتماعی آبراه
  • سرحلقه صالحین پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری
  • عضو سازمان بسیج اساتید دانشگاهها و مراکز آموزش عالی استان چهارمحال و بختیاری
  • عضو بسیج اساتید دانشگاهها و مراکز آموزش عالی استان خوزستان  
  • مسئول آموزش (صالحین) بسیج اساتید دانشگاه صنعتی خاتم الانبیاء(ص) بهبهان
  • عضو کمیته مرکزی نانوتکنولوژی دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان
  • رئیس کارگروه بررسی توانایی های علمی هیات اجرایی جذب اعضاء هیات علمی
  • عضو سازمان جوانان جمعیت هلال احمر
  • همکاری با شورای نگهبان دفتر بازرسی و نظارت بر انتخابات شهرستان بروجن
  • عضو و رئیس شورای عالی کانون بسیج اساتید دانشگاه صنعتی خاتم الانبیاء(ص) بهبهان (منصوب طی فرایند انتخابات و حکم مسئولین(
  • جانشین بسیج اساتید دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان (منصوب طی فرایند انتخابات و حکم مسئولین(
  • مشارکت در طراحی، راه اندازی و برگزاری کارگاههای فرهنگی ضمن خدمت
  • عضو شورای عالی بسیج اساتید دانشگاهها و مراکز آموزش عالی استان خوزستان (منصوب طی فرایند انتخابات و حکم مسئولین)
  • مسوول فضای سبز شهرداری بروجن.
  • مدد کار ترویجی سازمان جهاد کشاورزی استان چهارمحال و بختیاری.
  • مهندس ناظر طرح گندم شهرستان بروجن.
  • مشاور علمی شرکت مهتدسی نگین خاک در انجام مطالعات توانایی ریزسازواره های موجود در محدوده پروژه تغذیه مصنوعی دوراهان در زیست پالایی آلودگی های زیست محیطی آن
  • مشاور شرکت مهندسین مشاور تهران در مطالعات تجزیه زیستی آلودگی های خاک و آب پروژه تغذیه مصنوعی دشت خانمیرزا
  • مشاور عالی شرکت مهندسی نگین آب در ارزیابی عوامل و روشهای زیست پالایی آلودگی  های زیست محیطی سد زاینده رود

 سوابق آموزشی:

  • تدريس دردانشگاه پيام نوربروجن درسطح کارشناسی.
  • تدریس در دانشگاه صنعتی خاتم الانبیاء(ص) بهبهان، دروس ژنتیک عمومی، آزمایشگاه ژنتیک، بیوشیمی عمومی، آزمایشگاه بیوشیمی، زیست سلولی مولکولی، آزمایشگاه سلولی مولکولی، ویروس شناسی، میکروبیولوژی عمومی، آزمایشگاه میکروبیولوژی، ژنتیک جنگل، آزمایشگاه ژنتیک جنگل، ایمونو بیولوژی در سطح کارشناسی
  • تدریس در دانشگاه شهرکرد، دروس بیوشیمی 2، میکروبیولوژی عمومی، ویروس شناسی، ایمنی زیستی، بیوشیمی عمومی در سطح کارشناسی
  • ندریس در دانشگاه شهرکرد، دروس بیوتکنولوژی محیط زیست در سطح کارشناسی ارشد

 سوابق پژوهشی:

2018 

Jun 2014

 Evaluation of Antibacterial, Antifungal, and Antioxidant Properties of  Narcissus Bulbs from Behbahan

 Virtual screening for finding potential drug like chemicals which blocks extracellular domain of CD 47 as an anti-cancer target

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology

Behbahan, Iran

Finding a chemical inhibitor which prevents Ebola virus entrance

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology

Behbahan, Iran

Effect of some factors on embryogenesis and regeneration in maize (Zea mays L.) anther culture

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology

Behbahan, Iran

2015

Estimating the amount of secreted recombinant protein A using Fussy Regression insight into; determination of the optimal production conditions

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology, Behbahan, Iran

2015

Listeriolysin inhibition and drug design

Vira Vigene research and technology institute,

2015

Molecular docking based screening of predicted potential inhibitors for VP40 from Ebola virus

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology, Behbahan, Iran

2015

In-silico inhibition of Sortase from Listeria monocytogenes as a drug design tool

Vira Vigene research and technology institute,

2015

Finding a new compound inhibitor of amyloid-beta protein with a drug model in order to inhibit the Alzheimer's disease

Vira Vigene research and technology institute,

Jun 2015

In-silico inhibition of Sortase from Listeria monocytogenes as a drug design tool

Vira Vigene research and technology institute,

Jun 2014

Virtual screening following molecular dynamics simulation for finding potential

inhibitors of enterotoxin B from Staphylococcus aureus

Behbahan  Khatam AlanbiaUniversity of Technology, Department of Biology

Behbahan, Iran

Aug 2012 – May 2013

PhD Student

Tarbiat Modares University, Department of Genetics (Faculty of Biological Sciences)

Tehrān, Iran

Mar 2012 – present

Surface display of protein A on spore and vegetative cells of Bacillus subtilis, E. coli and chitosan later use as a biotechnological product

National Institute of Genetic Engineering and Biotechnology, Department of Industrial and Environmental Biotechnology

Tehrān, Iran

Feb 2011 – present

High level recombinant protein A production and detection of its effects on immune systeme

National Institute of Genetic Engineering and Biotechnology, Department of Molecular Genetics

Tehrān, Iran

Aug 2010 – present

Recombinant vaccine production project against Hyalomma anatolicum anatolicom

Razi Vaccine and Serum Research Institute, Department of Biotechnology

Karaj, Iran

Jul 2009

Recombinant influenza vaccine production project

Razi Vaccine and Serum Research Institute, Department of Biotechnology

Karaj, Iran

2011

The effect of Iranian medicine and lactobacillus supernatant on inhibition of Staphylococcus aureus sortase as a potential drug target (Seminar)

National Institute of Genetic Engineering and Biotechnology, Department of Molecular Genetics

Tehrān, Iran

2011

Protein A : Generation and Applications (Seminar)

National Institute of Genetic Engineering and Biotechnology, Department of Molecular Genetics

Tehrān, Iran

2011

Assisting in the research project of Sortase in vitro analysis and inhibition as a potential drug target Iranian medicine and lactobacillus supernatant

National Institute of Genetic Engineering and Biotechnology, Department of Molecular Genetics

Tehrān, Iran

2011

Assisting in the national research project of Invitro and invivo evaluation of antibacterial potential of Lactobacillus plantarum HKN 01 isolated from Iranian traditional dairy products

National Institute of Genetic Engineering and Biotechnology, Department of Molecular Genetics

Tehrān, Iran

2011

Assisting in the research project of Gene Isolation, Cloning, expression and characterization of a novel chitinase gene of Streptomyces sp.BJ03 (isolated from Caspian sea); An insight into its role in biological control

National Institute of Genetic Engineering and Biotechnology, Department of Molecular Genetics

Tehrān, Iran

2010

Assisting in administrating the project of investigating the effect of dearth on 12 varieties of Bermuda grass

Boroujen municipality, under supervision of Dr.Khorshid Razmjoo, lecturer of Isfahan University of Technology

2010

Administrating the study of investigating the effect of dearth on 10 varieties of Zoshia grass

Boroujen municipality, under supervision of Dr.Khorshid Razmjoo, lecturer of Isfahan University of Technology

2010

Administrating the study of investigating the effect of saltiness on 12 varieties of Bermuda grass

Boroujen municipality, under supervision of Dr.Khorshid Razmjoo, lecturer of Isfahan University of Technology

2010

Administrating the study of investigating the effect of saltiness on 10 varieties of Zoshia grass

Boroujen municipality, under supervision of Dr.Khorshid Razmjoo, lecturer of Isfahan University of Technology

2010

In vitro Tissue culture of Fritillaria imperialis (Seminar)

Tehran PNU, under supervision of Dr.Mohammad jafaraghayi, assistance professor of Seed and Plant Improvement Institute.

2010

Administrating the study of investigating the effect of saltiness on 12 varieties of Bermuda grass

Boroujen municipality, under supervision of Dr.Khorshid Razmjoo, lecturer of Isfahan University of Technology

2009

Acting as an assistant in the research project of the effect of aerating on the rice root in Hydroponic system

Under the supervision of Dr.Aghafakhr Mirlohi , the professor of Isfahan University of Technology

2009

Assisting in the national research project of improving the quality & quantity of Triticum turgidum l grain

Under the supervision of Dr. Ahmad arzani, the professor of Isfahan University of Technology.

2009

Cooperating in the research project of the outcomes of drought in Chaharmahal o Bakhtiari province in order to suggest the drought and salt resistant plant species

Shahrekord center of water – related studies

2009

Preparing the optimum utilizing plan of Siasard forest park

Boroujen municipality

2009

Preparing the optimum utilizing plan of Ahmadabad forest park

Boroujen municipality

2009

Preparing the complete plan of Boroujen area under the title of: “Green area the breathing lungs of the city “

Boroujen municipality

2009

Preparing the well organizing plan of Boroujen green area

Boroujen municipality

2009

Preparing the identity papers of Boroujen green area

Boroujen municipality

2009

Preparing the green house plan (designing, administration, care and economic justifications)

Boroujen municipality

2009

Preparing the women park plan of Boroujen

Boroujen municipality

2009

Preparation of the proposal of the isolation project of the green area water from drinkable water in Boroujen and management and supervising on preparing and administrating of the mentioned project after getting it approved

Boroujen municipality

2009

Suggesting the proposal for the wastes management, preparation, supervision and administrating after its approval

Boroujen municipality

 

  مقالات مجلات:

 

Garshasb Rigi Samira Ghaedmohammadi Gholamreza Ahmadian: A comprehensive review on staphylococcal protein A (SpA): Its production and applications. Biotechnology ans Applied Biochemistry. 66 (3):454-464 (2019)

Mostafa Akbariqomi, Reza Heidari, Soraya Saleh Gargari, Mir Davood Omrani, Garshasb Rigi, Nafiseh Sadat Sanikhani, Hamid Kooshki, Fatemeh Mahmoudian, Mohammad Ali Mazlomi, Gholamreza Tavoosidana1: Evaluation and statistical optimization of a method for methylated cell-free fetal DNA extraction from maternal plasma. Journal of Assisted Reproduction and Genetics. 36 (5): 1029-1038 (2019).

Raham Armand, Garshasb Rigi, Tahereh Bahrami: Fuzzy Hybrid Least-Squares Regression Approach to Estimating the amount of the Extra Cellular Recombinant Protein A from Escherichis coli BL21. Scientific Journal of Ilam University of Medical sciences, 2019

Nezam Armand, Raheleh Ahmadpour, Saeedreza Hosseinzadeh, Garshasb Rigi: The effect of compost on some photosynthetic parameters in three growth stages of lentil (Lens culinaris Medik) under drought stress. Journal of Plant Researches. 31(4): 916-926 (2019).

Raheleh Ahmadpour; Nezam Armand; Saeed Reza Hosseinzadeh; Garshasb Rigi: Review the use of compost fertilizer in soil and its influence on antioxidant activity, physiological and biochemical characteristics of lentil (Lens culinaris Medik) under water stress. Iranian journal of Pulses Research. 10 (1): 141-154 (2019)

Parisa Ghahremanifard, Nasim Rezaeinezhad, Garshasb Rigi, Fatemeh Ramezani, Gholamreza Ahmadian: Designing a novel signal sequence for efficient secretion of Candida antarctica lipase B in E. coli: The molecular dynamic simulation, codon optimization and statistical analysis approach. International Journal of Biological Macromolecules. 119 : 291-305 (2018)

Neda Ebrahimzadeh, Hajar Vaseghi, Garshasb Rigi, Gholamreza Esmaeeli Djavid, Shahla Mohammad Ganji, Amirreza Farhoud, Majid Pornour: DRD2 Dopamine Receptor and IL-1β Gene Expression Changes Involved in Wound Healing in Type 2 Diabetic Patients with Foot Ulcer. Journal of Mazandaran University of Medical Sciences. 28 (162) :47-58 (2018)

Raham Armand, Garshasb Rigi, Reza Alizadeh: Removal of Green 6 Direct Dye from Aqueous Solutions Using Immobilized Laccase Enzyme on Zinc Ferrite Nanoparticle. Journal of Rafsanjan University of Medical Sciences. 16 (9): 857-868 (2018)

Hajar Vaseghi, Majid Pornour, Gholamreza Esmaeeli Djavid, Garshasb Rigi, Shahla Mohammad Ganji, Leila Novin: Association of the gene expression variation of tumor necrosis factor-α and expressions changes of dopamine receptor genes in progression of diabetic sever foot ulcer. Iranian Journal of Basic Medical Sciences. 20(11): 1219-1213 (2017).

Ibrahim Torktaz, Jafar Hemmat, Ali Asghar Karkhane, Garshasb Rigi, Amin Rostami, Jafar Khezri, Reza Behroozi: Molecular Engineering of the Geobacillus stearothermophilus α-Amylase and Cel5E from Chlostridium thermocellim; In Silico Approach. Iranian Journal of Biotechnology (IJB). 16(3): 185-192 (2018).

Garshasb Rigi, Mohsen Farhadpour, Alireza Ghassempour, Gholamreza Ahmadian, Hassan Y. Aboul-Enein : Per Aqueous Liquid Chromatography (PALC) as a Simple Method for Native Separation of Protein A. Chromatographia. 80(11): 1633-1639 (2017)

Simin Khodaei, Mehdi Mohammadi, Samira Ghaedmohammadi, Garshasb Rigi, Reza Zadmard, Gholamreza Ahmadian: Covalent immobilization of protein A on chitosan and aldehyde double-branched chitosan as biocompatible carriers for IgG purification. )under review).

Garshasb Rigi, Ibrahim Torktaz, Ahmad Ismaili, Abbas Mohammadi Azhar: A novel inhibitor of Staphylococcus aureus enterotoxin B, an in silico approach.)under review).

Sara Ghafari, Matin Komeilian, Mohaddese sadat Hashemi, Sareh Oushani, Garshasb Rigi, Behnam Rashidieh, Kamran Yarahmadi, Fatemeh Khoddam: Molecular docking based screening of Listeriolysin-O for improved inhibitors. Bioinformation 13(5): 160-163 (2017)

Abbas Shali, Garshasb Rigi, Majid Pornour, Gholamreza Ahmadian: Expression and secretion of Cyan Fluorescent Protein (CFP) using chitinase promoter of Bacillus pumilus SG2 in B. subtilis. Iranian Biomedical Journal (IBJ) 21(4): 240-248 (2017)

Garshasb Rigi, Mohammad Vala Ashdar Nakhaei, Hoda Eidipour, Arshia Najimi, Fahimeh Tajik, Niloufar Taher, Kamran Yarahmadi: Virtual screening following rational drug design based approach for introducing new anti-amyloid beta aggregation agent. Bioinformation 13(2): 31-36 (2017)

Garshasb Rigi, Raham Armand, Ibrahim Torktaz: Ligand screening approach to find potential inhibitors of GP1 from Ebola Virus. Journal of Paramedical Sciences (JPS) 8(2): 42-45 (2017)

Garshasb Rigi, Raham Armand, Tahereh Bahrami: Estimation of the Amount of Recombinant Protein A Secretion Using Fuzzy Regression. Iranian Journal of Health Sciences 4 (3), 35-44 (2016)

Behnam Rashidieh, Zohreh Madani, Mahtab Khoshnejad Azam, Saeedeh Khalesi Maklavani, Newsha Ramezani Akbari, Shaghayegh Tavakoli, Garshasb Rigi: Molecular docking based virtual screening of compounds for inhibiting sortase A in L. monocytogenes. Bioinformation 11 (11), 501-505 (2015)

Kambiz Morabbi Heravi, Garshasb Rigi, Maryam Rezaei Arjomand, Amin Rostami, Gholamreza Ahmadian: An alternative bacterial expression system using Bacillus pumilus SG2 chitinase promoter. Iranian Journal of Biotechnology 13 (4), 17-23 (2015)

Seyedeh Zahra Hosseini, Payam Pour Mohammadi, Garshsb Rigi: Effect of induction medium on embryogenesis of maize anther culture and increase of normal haploid plantlet regeneration with wathman filter paper. Iranian Journal of Plant Physiology 5 (4), 1505- 1512 (2015)

Garshasb Rigi, Parisa Beyranvand, Samira Ghaedmohammadi, Sara Heidarpanah, Kambiz Akbari Noghabi, Gholamreza Ahmadian: Comparison of the extracellular full-length and truncated recombinant protein A production in Escherichia coli BL21 (DE3). Journal of Paramedical Sciences (JPS) 6(3): 37-47 (2015)

Danya Abazari, Mehrad Moghtadaei, Ali Behvarmanesh, Bahareh Ghannadi, Monireh Aghaei, Mahboobeh Behruznia, Garshasb Rigi: Molecular docking based screening of predicted potential inhibitors for VP40 from Ebola virus. Bioinformation 11(5): 243-247 (2015)

Samira Ghaedmohammadi, Garshasb Rigi, Reza Zadmard, Ezio Ricca, Gholamreza Ahmadian: Immobilization of Bioactive Protein A from Staphylococcus aureus (SpA) on the Surface of Bacillus subtilis Spores. Molecular Biotechnology 2015 Apr; DOI: 10.1007/s12033-015-9868-z

Garshasb Rigi, Samira Ghaed Mohammadi, Maryam Rezaei Arjomand, Gholamreza Ahmadian, Kambiz Akbari Noghabi: Optimization of extracellular truncated Staphylococcal Protein A (SpA) expression in Escherichia coli BL21 (DE3). Biotechnology and Applied Biochemistry 2014 Mar; 61(2):217-25;

Majid Vahed, Ebrahim Motalebi, Garshasb Rigi, Kambiz Akbari Noghabi, Mohammad Reza Soudi, Mehdi Sadeghi, Gholamreza Ahmadian: Improving chitinolytic activity of Bacillus pumilus SG2 by random mutagenesis. Journal of Microbiology and Biotechnology 2013 Nov 28; 23(11):1519-28;

Farideh Zakeri, Majid Sadeghizadeh, Mohammad Reza Kardan, Hossein Shahbani Zahiri, Gholamreza Ahmadian, Fatemeh Masoumi, Hakimeh Sharafi, Garshasb Rigi, Hojatollah Vali, Kambiz Akbari Noghabi: Differential proteome analysis of a selected bacterial strain isolated from a high background radiation area in response to radium stress. Journal of proteomics 05/2012; 75(15):4820-32.

H Sharafi, L Alidost, H Abassi, G Rigi, H Shahbani Zahiri, G Ahmadian, H Vali, K Akbari Noghabi: Antibacterial activity of probiotic Lactobacillus plantarum HK01: Effect of divalent metal cations and food additives on production efficiency of antibacterial compounds. Preparative Biochemistry & Biotechnology 01/2012;

Garshasb Rigi, Gholam Ali Kardar, Kambiz Akbari Noghabi, Gholamreza Ahmadian: Detection of protein A effects on immune system. Iranian journal of Immunology; 05/2014; 11: 786-787

Garshasb Rigi, Morteza Esfahanian, Khosrow Aghayipur, Reza Behrouzi, Gholamreza Ahmadian: Cloning and Sequencing of HAO3 gene of Hyalomma anatolicum anatolicum in unsecretory shuttle vector pPICZB. Modern Genetics Journal; 05/2012

 

کتابها:

کتب علمی:

  • معرفی فلوسایتومتری، ترجمه، دکتر گرشاسب ریگی چشمه علی، رضا حیدری، دکتر حسین بختو،انتشارات جهاد دانشگاهی علوم پزشکی تهران.
  • Real-time PCR، ترجمه، دکتر گرشاسب ریگی چشمه علی، رضا حیدری، دکتر حسین بختو،انتشارات جهاد دانشگاهی علوم پزشکی تهران.
  • اصول و روشهای پرکاربرد آزمایشگاهی زیست شناسی مولکولی و سلولی و تکنیکهای مهندسی ژنتیک، تدوین، دکتر گرشاسب ریگی چشمه علی، دکتر حسین بختو،انتشارات جهاد دانشگاهی علوم پزشکی تهران.
  • خالص سازی پروتئینهای چالش برانگیز، تدوین، دکتر گرشاسب ریگی چشمه علی، محسن فرهادپور، دکتر حسین بختو،انتشارات جهاد دانشگاهی علوم پزشکی تهران.

کتب فرهنگی:

 

اختراعات:

  • High level extracellular recombinant protein A production (filing)
  • Development of simple and economical method for purifying protein A (SpA) using hydrophilic interaction liquid chromatography technique (filing)

ژنهای شناسایی شده، توالی یابی شده و ثبت شده در بانکهای ژن بین المللی، Gene Bank در National Center for Biotechnology Information (NCBI) ،  ENAدر اروپا و DNA Data Bankدر ژاپن:

ACCESSION No: MH361761

Mycobacterium tuberculosis strain GR early secretory antigenic target 6 kDa protein (esxA) gene, complete cds.

              protein_id="AXL96670"

ACCESSION No: MH370480

Exiguobacterium sp. HA2 isocitrate dehydrogenase (citC) gene, partial cds.

protein_id="AXL96671"

ACCESSION No: MH378445

Exiguobacterium sp. HA2 DNA-directed RNA polymerase beta (rpoB) gene, partial cds.

protein_id="AXL96672"

ACCESSION No: MH378775

Exiguobacterium sp. HA2 class I heat shock protein (hsp70) gene, partial cds.

protein_id="AXL96673"

ACCESSION No: MH379139

Exiguobacterium sp. HA2 universal major cold shock protein (csp) gene, complete cds.

protein_id="AXL96674"

ACCESSION No: MH325952

Hyalomma anatolicum anatolicum HAO3 (HAO3) gene, complete cds

              protein_id="AXL96675"

ACCESSION No: MH379633

Narcissus tazetta L. cv. Shahla-e- Behbahan norbelladine 4'-O-methyltransferase (N4OMT4) mRNA,  complete cds.

              protein_id="AXL96676"

ACCESSION No: MH388024

Synthetic construct hemagglutinin (HA) gene, partial  cds.

              protein_id="AWW21439"

ACCESSION No: MH368127

Synthetic construct segment 4 hemagglutinin (HA) gene, complete cds.

              protein_id="AWL07886.1"

ACCESSION No: MH360730

Influenza A virus (A/chicken/Karaj/GHA/2017(H9N2)) segment 4 hemagglutinin (HA) gene, complete cds.              protein_id="AWK91180.1"

ACCESSION No: MH360730

Influenza A virus (A/chicken/Karaj/GM2/2017(H9N2)) segment 7 matrix protein 2 (M2) and matrix protein 1 (M1) genes, complete cds.

proteins_id="AWK24095.1", "AWK24094.1"

جوایز و افتخارات:

  • کسب بالاترین امتیاز پژوهشی سال 1394 دانشگاه صنعتی خاتم الانبیاء(ص) بهبهان (رتبه اول دانشگاه – پژوهشگر برتر دانشگاه - در امتیاز بندی هفته پژوهش)
  • برگزیده به عنوان پژوهشگر برتر دانشکده علوم پایه دانشگاه صنعتی خاتم الانبیاء(ص) بهبهان (رتبه اول سال 94)
  • برگزیده به عنوان استاد پژوهشگر نمونه بسیجی دانشگاه صنعتی خاتم الانبیاء(ص) بهبهان
  • دریافت گواهی آموزشی  آکادمی  بین المللی TUV انگلستان
  • دریافت جایزه و گواهینامه آکادمی بین المللی PCMS استرالیا در سومین همایش سراسری محیط زیست، انرژی و پدافند انرژی
  • كسب عنوان نخبه برتر در جشنواره تجلي خود باوري علمي ونو آوري
  • كسب رتبه دانشجوي ممتاز دوره کارشناسی ارشد
  • موفقیت دردریافت بورس کامل تحصیلی درسطح دکتری داخل از وزارت علوم، تحقیقات وفناوری
  • موردتجلیل در جشنواره جهاد گران علم وفناوری سازمان بسیج علمی پژوهشی
  • کسب عنوان دانشجوی ممتاز و برجسته پژوهشی در دوره دکتری تخصصی پژوهشگاه ملی مهندسی ژنتیک و بیوتکنولوژی
  • موفقیت در مبارزه وکنترل آفات وبیماریهای همه گیرمنطقه بروجن واخذ لوح تقدیرازمدیریت جهاد کشاورزی شهرستان بروجن.
  • موفقیت درمدیریت بحران آب وخشک سالی وهمچنین ستاد حوادث غیر مترقبه شهرداری واخذ تقدیرنامه های مربوطه.
  • برگزیده به عنوان استاد نمونه بسیجی و فرمانده برتر جنگ نرم
  • مورد تجلیل توسط شهردار و شورای اسلامی شهر بروجن به دلیل برجستگی در مدیریت و برنامه ریزی شهری

 

 سایر موارد:

تدریس در کارگاه های آموزشی:

  • Stereotactic surgical techniques in laboratory animals, Western blotting basic techniques of molecular and cell culture techniques workshop instructor at 3rd. International Congress of Biology and Ecology
  • کارگاه آموزشی GLP در دانشگاه صنعتی خاتم الانبیاء بهبهان
  • کارگاه آموزشی با عنوان بیوانفورماتیک و طراحی دارو برگزار شده در اولین کنفرانس تخصصی زمینه های شغلی و پژوهشی در عرصه طراحی دارو کشور در محل تالار همایشهای وزارت کشور زیر نظر مرکز مطالعات و توسعه پژوهش های زیست فناوری.
  • کارگاه آموزشی با عنوان فرصت های مطالعاتی و در آمد زایی در زمینه بیوتکنولوژی دارویی برگزار شده در اولین جشنواره سمپوزیوم های آموزشی کارآفرینی در علوم زیست فناوری کشوردر محل تالار همایشهای وزارت کشور زیر نظر مرکز مطالعات و توسعه پژوهش های زیست فناوری.
  • کارگاه آموزشی با عنوان فرصت های مطالعاتی و در آمد زایی در زمینه ژن درمانی و سلول درمانی برگزار شده در اولین جشنواره سمپوزیوم های آموزشی کارآفرینی در علوم زیست فناوری کشوردر محل تالار همایشهای وزارت کشور زیر نظر مرکز مطالعات و توسعه پژوهش های زیست فناوری.
  • کارگاه آموزشی با عنوان فرصت های مطالعاتی و در آمد زایی در زمینه سرطان برگزار شده در اولین جشنواره سمپوزیوم های آموزشی کارآفرینی در علوم زیست فناوری کشوردر محل تالار همایشهای وزارت کشور زیر نظر مرکز مطالعات و توسعه پژوهش های زیست فناوری.
  • کارگاه آموزشی تئوری و عملی مهندسی ژنتیک (Genetic Engineering ) در جهاد دانشگاهی علوم پزشکی ایران، واحد دانشگاه علوم پزشکی تهران
  • کارگاه آموزشی تئوری و عملی کلونینگ و تکنیکهای مولکولی(Cloning and Molecular techniques )در جهاد دانشگاهی علوم پزشکی ایران، واحد دانشگاه علوم پزشکی تهران
  • کارگاه آموزشی تئوری و عملی Real-Time RCR در جهاد دانشگاهی علوم پزشکی ایران، واحد دانشگاه علوم پزشکی تهران
  • کارگاه آموزشی تئوری و عملی طراحی پرایمر و اصول PCR (Primer Design and PCR )در جهاد دانشگاهی علوم پزشکی ایران، واحد دانشگاه علوم پزشکی تهران
  • کارگاه آموزشی تئوری و عملی بیان ژن و تولید پروتئین های نوترکیب (Gene Expression and Recombinant Protein Production ) در جهاد دانشگاهی علوم پزشکی ایران، واحد دانشگاه علوم پزشکی تهران
  • کارگاه آموزشی بیوانفورماتیک ژنومیکس دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان
  • کارگاه آموزشی بیوانفورماتیک پروتئومیکس دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان
  • کارگاه آموزشی نرم افزار  Endnote دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان
  • کارگاه آموزشی طراحی و تجزیه و تحلیل آزمایشات میکروبیولوژی با استفاده از روش Response Surface Methodology (RSM) و نرم افزارهای Design Expert وSigma plat 

شرکت در کارگاه های آموزشی:

  • کارگاه آموزشی با عنوان نسل جدید داروهای نوترکیب برگزار شده در اولین کنفرانس تخصصی زمینه های شغلی و پژوهشی در عرصه طراحی دارو کشور در محل تالار همایشهای وزارت کشور زیر نظر مرکز مطالعات و توسعه پژوهش های زیست فناوری.
  • کارگاه آموزشی با عنوان نسل آینده داروهای زیستی برگزار شده در اولین کنفرانس تخصصی زمینه های شغلی و پژوهشی در عرصه طراحی دارو کشور در محل تالار همایشهای وزارت کشور زیر نظر مرکز مطالعات و توسعه پژوهش های زیست فناوری.
  • کارگاه آموزشی روش تحقیق در دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان، هفته پژوهش
  • کارگاه آموزشی ناهنجاری های اسکلتی و حرکات اصلاحی در دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان، هفته پژوهش
  • کارگاه آموزشی دانشگاه آموزش محور- دانشگاه پژوهش محور در دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان، هفته پژوهش
  • کارگاه آموزشی مقاله نویسی در دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان، هفته پژوهش
  • کارگاه آموزشی معرفی پتنت و استفاده از آن در پروپوزال و پایان نامه در دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان، هفته پژوهش
  • کارگاه آموزشی مقررات و قوانین ارتقاء اعضاء هیات علمی در دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان، 
  • شرکت درچهارمین کنگره علوم زراعت واصلاح نباتات ایران . کرج موسسه تحقیقات اصلاح وتهیه نهال و بذر.
  • شرکت درششمین کنگره علوم زراعت واصلاح نباتات ایران . دانشگاه مازندران بابلسر .
  • شرکت درهفتمین کنگره علوم زراعت واصلاح نباتات ایران . کرج موسسه تحقیقات اصلاح و تهیه نهال و بذر .
  • شرکت در دوره مدیریت فضای سبزشهری درسومین همایش ملی فضای سبزومنظرشهری جزیره کیش ودریافت گواهینامه از سازمان شهرداریها و دهیاریهای کشور .
  • شرکت در دوره مدیریت آبیاری و بحران خشکی در فضای سبز دراصفهان ودریافت گواهینامه ازسازمان شهرداریها و دهیاریهای کشور و سازمان پارکها و فضای سبز اصفهان
  • شرکت دردوره مدیریت پسماند دراصفهان ودریافت گواهینامه ازسازمان شهرداریها ودهیاریهای کشور و سازمان پسماند اصفهان .
  • شرکت در دوره مدیریت پسماند در شیراز و دریافت گواهینامه ازسازمان شهرداریها و دهیاریهای کشور و سازمان پسماند شیراز .
  • شرکت در پنجمین همایش ملی مدیریت پسماند مشهد .
  • دریافت گواهینامه پرورش و تولید گلهای زینتی شاخه بریده ازمجتمع آموزش جهاد کشاورزی استان چهارمحال و بختیاری .
  • شرکت در کارگاه تئوری و عملی Good Laboratory Practice (GLP)در پژوهشگاه ملی مهندسی ژنتیک و بیوتکنولوژی
  • شرکت در کارگاه آموزشی- ترویجی نانو فناوری دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان

 عضویت در مجامع علمی:

  • عضو انجمن ژنتیک ایران
  • عضو انجمن بیوتکنولوژی ایران
  • عضو انجمن بیو انفورماتیک ایران
  • عضو انجمن ایمنی زیستی ایران
  • عضو انجمن ایمنولوژی و آلرژی ایران
  • عضو شبکه بیوتکنولوژی و ژنتیک سلامت شرق مدیترانه (Eastern Mediterranean Health Genomics and Biotechnology Network (EMGEN) )
  • عضویت در کانون گسترش فضای سبز و حفظ محیط زیست ایران
  • عضویت درسازمان نظام مهندسی کشاورزی ومنابع طبیعی استان چهارمحال و بختیاری
  • مسوول کمیته علمی پژوهشی گروه زراعت واصلاح نباتات دانشکده کشاورزی دانشگاه صنعتی اصفهان
  • عضو انجمن علمی زیست شناسی پژوهشگاه ملی مهندسی ژنتیک و بیوتکنولوژی
  • عضو کمیته مرکزی نانوتکنولوژی دانشگاه صنعتی خاتم الانبیاء(ص) بهبهان
  • عضویت درکانون نخبگان بسیجی استان چهارمحال و بختیاری
  • عضو انجمن علمی جنین شناسی و بیولوژی تولید مثل ایران

Iranian Society of Embryology & Reproductive Biology (ISERB)

داوری های علمی:

  • داوری مقالات در مجله Preparative Biochemistry & Biotechnology (ISI)
  • داوری مقالات در مجله Organic and Medicinal Chemistry Letters (ISI)
  • داوری مقالات در مجله Inflammation (ISI)
  • داور مجله( (ISI Journal, Elsevier Gene Reports
  • داوری در مجله Biotechnic & Histochemistry
  • داوری مقاله در مجله Eurasian Journal of Medicine (ISI journal)
  • داوری مقالات در مجله Journal of Cellular Biochemistry (ISI Journal, Wiley)
  • داوری مقالات در مجله( Photochemistry and Photobiology(ISI Journal, Wiley
  • داوری مقالات در مجله ((ISI (Asian Pacific Journal of Cancer Prevention (APJCP
  • داوری علمی مجله Journal of Applied Biotechnology Reports
  • داور مجله Journal of Immunology and Serum Biology
  • داور مجله Sky Journal of  Medicine and Medical Sciences (SJMMS)
  • داور مجله  Journal of Advances in Biology
  • داور مجله International Journal of Diabetes in Developing Countries
  • داوری مقالات در مجله علمی پژوهشی سلول و بافت
  • داوری مقالات در مجله علمی پژوهشی دانشگاه علوم پزشکی ایلام
  • داوری مقاله در مجله علمی دانشگاه علوم پزشکی فسا
  • داوری مقاله در مجله علمی پژوهشی دانشگاه علوم پزشکی شهید صدوقی یزد
  • داوری مقالات در مجله علمی پژوهشی بیماری های پستان ایران
  • داور و عضو کمیته علمی دومین کنگره بین المللی و چهاردهمین کنگره ملی علوم زراعت و اصلاح نباتات ایران
  • داور چهارمین کنفرانس بیوانفورماتیک ایران
  •  داوراولین جشنواره ونمایشگاه ملی اختراعات وابتکارات ایران
  • داور علمی اولین کنگره ملی زیست شناسی و علوم طبیعی ایران
  • داور علمی اولین کنفرانس بین المللی یافته های نوین در علوم کشاورزی، منابع طبیعی و محیط زیست
  • داور علمی اولین کنفرانس بین المللی مهندسی محیط زیست
  • داور علمی و عضو کمیته علمی دومین کنگره بین المللی علوم کشاورزی و محیط زیست
  • داور علمی و عضو کمیته علمی دومین کنگره جامع ملی یافته های نوین زیست شناسی
  • داور علمی دومین همایش ملی کاربرد علوم و فناوریهای نوین در کشاورزی، منابع طبیعی و محیط زیست
  • داور علمی سومین همایش سراسری محیط زیست، انرژی و پدافند زیستی
  • داور علمی سومین همایش سراسری کشاورزی و منابع طبیعی پایدار
  • داوری مجله ISI برای Advancement of Chemical Sciences (ACS) 
  • داوری مقاله جهت مجله Journal of Biosciences and Medicines (JBM)
  • داوری مقاله مجله جهت The American Society of Photobiology, Wiley Online Library
  • داور علمی اولین همایش علمی پژوهشی زیست شناسی و علوم باغبانی ایران
  • داور علمی کنفرانس بین المللی محیط زیست و منابع طبیعی
  • داور علمی اولین کنگره علمی پژوهشی توسعه و ترویج علوم کشاورزی و منابع طبیعی و محیط زیست ایران
  • داور و عضو کمیته علمی و داوران سومین کنگره بین المللی زیست شناسی و اکولوژی
  • عضو Editorial Board مجله علمی پژوهشی Letters in Health & Biological Sciences
  • عضو Editorial Board مجله علمی پژوهشی Journal of Environment and Health Science
  • عضو Editorial Board مجله علمی پژوهشی Journal of Pharmacy & Pharmaceutics
  • عضو Editorial Board مجله علمی پژوهشی Journal of Immunology and Serum Biology
  • عضو Editorial Board مجله علمی پژوهشی Journal of Bioinformatics and Proteomics Review
  • عضو Editorial Board مجله علمی پژوهشی Journal of Stem Cell and Regenerative Biology
  • عضو Editorial Board مجله علمی پژوهشی    Journal of Marine Biology and Aquaculture
  • داور و عضو کمیته علمی اولین همایش ملی پدافند غیر عامل در بخشهای کشاورزی،منابع طبیعی و محیط زیست با رویکرد توسعه پایدار
  • داور و عضو کمیته علمی اولین همایش ملی پیشرفت ها و چالش ها در علوم، مهندسی و فناوری
  • داور و عضو کمیته علمی و داوران کنفرانس بین المللی یافته های نوین پژوهش در شیمی و مهندسی شیمی
  • داوری متعدد پایان نامه های دانشجویی
  • داوری طرحهای پژوهشی
  • داوری کتابهای علمی
  • داور و عضو کمیته علمی اولین کنگره ملی راهکار های دستیابی به  توسعه پایدار در بخشهای علوم و فناوری
  • داور و عضو کمیته علمی دومین کنگره ملی زیست شناسی و علوم طبیعی ایران
  • داور علمی و عضو کمیته علمی دومین همایش علمی پژوهشی زیست شناسی و علوم باغبانی ایران
  • داور و عضو کمیته علمی دومین کنفرانس بین المللی محیط زیست و منابع طبیعی
  • داور و عضو کمیته علمی چهارمین همایش سراسری کشاورزی و منابع طبیعی پایدار
  • داور و عضو کمیته علمی دومین کنفرانس بین المللی مهندسی محیط زیست
  • داور و عضو هیات علمی دومین کنفرانس بین المللی یافته های نوین در علوم کشاورزی، منابع طبیعی و محیط زیست
  • داور و عضو هیات علمی سومین کنفرانس بین المللی پژوهش های کاربردی در علوم شیمی، مهندسی شیمی و زیست شناسی
  • داور و عضو کمیته علمی کنفرانس بین المللی یافته های نوین پژوهشی در علوم، مهندسی و فناوری
  • داور و عضو کمیته علمی   سومین کنگره بین المللی علوم کشاورزی، مواد غذایی و محیط زیست
  • داور و عضو کمیته علمی سومین همایش محیط زیست و بحران های پیش رو
  • داور و عضو کمیته علمی  پنجمین همایش سراسری کشاورزی و منابع طبیعی پایدار
  • داور و عضو کمیته علمی سومین کنگره ملی زیست شناسی و علوم طبیعی ایران
  • داور و عضو کمیته علمی دومین کنفرانس بین المللی یافته های نوین پژوهشی در علوم، مهندسی و فناوری
  • داور و عضو کمیته علمی اولین همایش ملی مدیریت بحران،ایمنی ،بهداشت،محیط زیست و توسعه پایدار
  • داور و عضو کمیته علمی اولین کنفرانس بین المللی کاربرد تحقیق و پژوهش در علوم و مهندسی
  • داور و عضو کمیته علمی دومین کنفرانس بین المللی یافته های نوین پژوهشی در علوم، مهندسی و فناوری
  • داور و عضو کمیته علمی پنجمین همایش سراسری محیط زیست،انرژی و پدافند زیستی
  • داور و عضو کمیته علمی دومین همایش ملی پدافند غیر عامل در بخشهای کشاورزی،منابع طبیعی و محیط زیست با رویکرد توسعه پایدار

 

  • دبیر علمی مجله علمی پژوهشی فیزیولوژی و بیوشیمی گیاهی ایران

توصیه نامه های علمی:

  • دکتر غلامرضا بخشی خانیکی، استاد و مدیر گروه بیوتکنولوژی کشاورزی دانشگاه پیام نور تهران
  • دکتر آقافخر میرلوحی، استاد گروه زراعت و اصلاح نباتات دانشگاه صنعتی اصفهان
  • دکتر جمشید رزمجو، دانشیار گروه زراعت و اصلاح نباتات دانشگاه صنعتی اصفهان
  • دکتر احمد ارزانی، استاد گروه زراعت و اصلاح نباتات دانشگاه صنعتی اصفهان
  • دکتر محمد رضا خواجه پور، دانشیار گروه زراعت و اصلاح نباتات دانشگاه صنعتی اصفهان
  • دکتر حسین صمدی، استادیار و رئیس مرکز تحقیقات منابع آب دانشگاه شهرکرد
  • دکتر غلامرضا احمدیان، استادیار گروه صنعت و محیط زیست پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری
  • دکتر کامبیز اکبری، استادیار و مدیر بخش صنعت و محیط زیست پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری
  • دکتر محمد علی ملبوبی، دانشیار گروه بیوتکنولوژی گیاهی پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری
  • دکتر امیر موسوی، دانشیار گروه بیوتکنولوژی گیاهی پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری
  • دکتر علی اصغر کارخانه، دانشیار گروه صنعت و محیط زیست پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری
  • دکتر غلامعلی کاردر، استادیار مرکز تحقیقات آسم و آلرژی دانشگاه علوم پزشکی تهران

برخی نمرات قابل ذکر:

  • نمره آزمون زبان تافل iBt : 120/85
  • نمره آزمون زبان MSRT : 100/62
  • نمره پایان نامه کارشناسی ارشد: 20/19.25
  • درجه پایان نامه کارشناسی ارشد:  عالی
  • نمره آزمون جامع دکتری تخصصی: 20/19.38
  • نمره رساله دکتری تخصصی: 20/19.95

درجه رساله دکتری تخصصی: عالی

مهارتهای علمی تخصصی:

Genetic Analysis, Gel Electrophoresis, LC-MS, MOLDI-TOF MS, Protein Analysis, Chromatographic Techniques, Immunoassay Development, Protein Characterization, Promoter Analysis, Flow Cytometer, Bacterial Genetics, Molecular Genomics, Immunogenetics, Classical Genetics, Genetic Analysis, Analytical HPLC, Bradford Assay, SDS-PAGE, CTAB DNA extraction, Cell Culture, Tissue Culture, Cloning, Sequencing,DNA Amplification, ELISA, PCR, Western Blot, Dialysis, DNA, Electrophoresis, Enzymes, Immunoassay, Mass Spectrometry, Protein Engineering, Gene Expression, Recombinant DNA and Protein, Genomics, Proteomics, Bioinformatics and Computational Biology, Cell Culture Techniques, HPLC-MS, Spectrophotometer, Genomic PCR,Genomic DNA Isolation, Statistical Design, High-Performance Liquid Chromatography (HPLC), Microbiology, Industrial and Environmental Biotechnology, Scientific Writing,  Protein Chemistry, Transfection, RNA Isolation, RT-PCR, Cell Biology, Biochemistry,

know lots of bioinformaticsoftwares and sites, Sites or online softwares such as:, Pymol, Mascot, EMBL, MATCH, PDB, PIR, Pfam, KEGG, GMO, SRS, Ensemble, BLOCKS, PRINTS, PubMed, Proteomics, Tigers, smart, Sage, GEO, Entrez, map Viewer, EPD, EPO, USPTO,  PROSITE,  NEB Cutter, swiss-prot, patch,  UNIPROT, REACTOME, INTERPRO, PRODOM, RefSeq, Phyre, CDD, SignalP, MegAlign, UCSC, CDART, Primerselect, Unigene, MapDraw, AGRICOLA, AGRIS, SurfIP, Patent Lens, PSORT, SWISS-2DPAGE database, FindMod, iProclass, Gene Indices, SNPdb, CAMBIA, TIGRFAMs, Gene Ontology (GO), NCBI, BLAST, mFold, Clustal w2, I-TASSER, TargetP, peptide property calculator, ExPaSy, msproduct, eTBLAST,

 SAFTWARES such as:, Vector.NTI Advance, CLONEWIN, MEGA, Bioedith,  SeqMan, SPSS, OLIGO, Chromas, Genetyx, Protean, GeneMan, EditSeq, SnapGene Viewer, Gene Runner,  Molegro Molecular Viewer, Design – Expert, SigmaPlot, MagTran, Xcalibor, Spdbv,

 لینکهای تکمیلی رزومه:

https://www.researchgate.net/profile/Garshasb_Rigi3/

 http://www.  ir.linkedin.com/pub/garshasb-rigi-ph-d/48/365/ba2/

 http://garshasbbiotech.blogfa.com/

https://www.sku.ac.ir/en/DepartmentGroup/ProfessorForm.aspx?ID=2342

https://www.sku.ac.ir/~rigi#

https://scholar.google.com/citations?user=T6YVtr4AAAAJ&hl=en

ORCID ID: http://orcid.org/0000-0003-2450-912X

Scopus ID (Author ID): 55255171200

https://www.scopus.com/authid/detail.uri?authorId=55255171200 

Researcher ID :   L-9597-2016
Profile URL :   http://www.researcherid.com/rid/L-9597-2016

https://www.mendeley.com/profiles/garshasb-rigi/ 

publons.com/a/1568808/

Differential proteome analysis of a selected bacterial strain isolated from a high background radiat

Journal of Proteomics

Available online 22 May 2012


Differential proteome analysis of a selected bacterial strain isolated from a high background radiation area in response to radium stress

  • Farideh Zakeria, b, c, Majid Sadeghizadeha, Mohammad Reza Kardand, Hossein Shahbani Zahirib, Gholamreza Ahmadianb, Fatemeh Masoumib, Hakimeh Sharafib, Garshasb Rigib, Hojatollah Valie, Kambiz Akbari Noghabib, ,
  • a Department of Genetics, Faculty of Biological Science, Tarbiat Modares University, P.O. Box 14115-154, Tehran, Iranb Department of Molecular Genetics, National Institute of Genetic ngineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iranc Agriculture, Medicine and Industry Research School, Nuclear Science and Technology Research Institute, Tehran, Irand Radiation Applications Research School, Nuclear Science and Technology Research Institute- Iranian nuclear regulatory authority, Tehran, Irane Facility for Electron Microscopy Research, McGill University, 3640 Street, Montreal, Quebec, Canada H3A 2B2
  • Received 10 January 2012. Accepted 13 May 2012. Available online 22 May 2012.

Abstract

The present study describes the response of a bacterial strain, isolated from a hot spring in an area with the highest levels of natural radiation, under radium (226Ra) stress. The bacterium has been characterized as a novel and efficient radium biosorbent and identified as a variant of Serratia marcescens by biochemical tests and molecular recognition. In order to gain insights into key cellular events that allow this strain to survive and undergo 226Ra adaptation and biosorption, the strain was tested under two experimental conditions of 1000 and 6000 Bq 226Ra stress. A proteomic approach involving two-dimensional polyacrylamide gel electrophoresis and mass spectrometry was used to identify the differentially expressed proteins under 226Ra stress. Functional assessment of identified proteins with significantly altered expression levels revealed several mechanisms thought to be involved in 226Ra adaptation and conferring resistant phenotype to the isolate, including general stress adaptation, anti-oxidative stress, protein and nucleic acid synthesis, energy metabolism, efflux and transport proteins. It suggests that this strain through evolution is particularly well adapted to the high background radiation environment and could represent an alternative source to remove 226Ra from such areas as well as industrial radionuclide polluted wastewaters.

Keywords

Serratia marcescens; 226Ra stress; Proteomics; Biosorption; High background radiation area

Biological control of charcoal rot (Macrophomina phaseolina) using Streptomyces sp. BJ03 isolated fr

Biological control of charcoal rot (Macrophomina phaseolina) using Streptomyces sp. BJ03 isolated from North of Iran

Reza Behroozi1, Nesa Jafari1, Kambiz Akbari noghabi1, Gholamreza Ahmadian*1, Kosar Rahimzadeh1, garshaseb Rigi1 and Esmaeil Frozan1 1 National Institution of Genetic Engineering and Biotechnology (NIGEB) *Corresponding author: gholamrezaahmadian@yahoo.ca

Macrophomina phaseolina is a plant pathogenic fungus which have a broad range of host such as soybean, bean, chickpea, pine and sesame. This fungus is a soil born pathogen that infects its hosts in primary growth stages like seedling growth. In this study, antifungal activity against Macrophomina phaseolina, was examined and inhibition percentage values was calculated. According to activity and broad spectrum of isolates, three isolates showed a high activity against charcoal rot and one of them had the highest activity. Some morphologically appearance suggested that these species belong to genus Streptomyces and phylogenetic studies using 16SrDNA confirmed it. Morphological changes such as hyphal branching and abnormal shape were observed in fungi grown on ISP2 medium. Hence, Streptomyces sp. BJ03 highly produced extracellular chitinase on colloidal chitin agar plates, it seems that this antifungal activity of this strain is related to production of extracellular chitinase. Therefore, the selected strain could be used for biocontrol of phytopathogenic fungi.

Keywords: Macrophomina phaseolina , Streptomyces sp. BJ03, biological control

ning and Sequencing of HAO3 gene of Hyalomma anatolicum anatolicum in unsecretory shuttle vector pPI

Cloning and Sequencing of HAO3 gene of Hyalomma anatolicum anatolicum in unsecretory shuttle vector pPICZB

Garshasb Rigi Cheshmehali1, Morteza Esfahanian2, Khosrow Aghayipur3, Reza Behrouzi4, Nezam Armand 5 ,Gholamreza Ahmadian6*

1- PhD student in Molecular Genetics in National Institute of Genetic Engineering and Biotechnology 2- M.Sc in Biotechnology in Razi Vaccine and Serum Research Institute3- Assistant professor of Department of Biotechnology, Razi Vaccine and Serum Research Institute 4- M.Sc in Biotechnology in National Institute of Genetic Engineering and Biotechnology 5- Lecturer  of  Department of Biology in Behbahan Khatamolanbia University of Technology 6- Assistant professor of Department of  Molecular Genetics in National Institute of Genetic Engineering and Biotechnology (gholamrezaahmadian@yahoo.ca*)

Abstract

Introduction :Hyalomma anatolicum anatolicum is parasite of farm and wild animals in tropical and sub tropical region of the world include south of Iran .Ticks of genus Hyalomma are vector of many disease also this parasite cause decrease of milk, meat and low quality leaser which produce by its host.

Objective: HAO3 gene is candid to invent new vaccine against this tick.

Material and Methods: In this study we use two ORF specific primers to amplify HAO3 in Termocycler then it has ligated with pPICZB expression vector and transformed in E.Coli DH5α then pPICZB has extracted from E.Coli .on next stage HAO3 has made apart from pPICZB by two restriction enzyme (EcoRI and XbaI).

Results: Sequencing shows that our gene is exactly like the HAO3 which is in gene bank and it is partly like BM86 and HA86.

 

Keywords: HAO3 gene, cloning, sequencing, vaccine

The Role Of NMDA Receptors In Tolerance And Dependence To The Morphine In The Striatum And The Prefr

The Role Of NMDA Receptors In Tolerance And Dependence To The Morphine In The Striatum And The Prefrontal Cortex Of Rat Brain

 

Fatemeh Rafieenia1*, Shamseddin Ahmadi2, Jalal Rostamzadeh2, Sabrieh Amini3, Garshasb Rigi Cheshmehali4

 

1M.Sc in Cellular and Molecular Biology, University of Kurdistan, Sanandaj, Iran

Assistant professor of  Department of Biological Science & Biotechnology, Faculty of Science, University of Kurdistan, Sanandaj, Iran

Assistant professor  of  Department of Biological, Faculty of Science, Islamic Azad University, sanandaj branch, iran

4 PhD student in Molecular Genetics in National Institute of Genetic Engineering and Biotechnology, Tehran, iran

 

Introduction: N-Methyl-D-Aspartate (NMDA) receptors have important roles in learning, memory formation, tolerance and dependence to the morphine.

Aim: In the present study, after induction of morphine dependence in male wistar rats, changes in gene expression of NR1 subunit of NMDA receptors, the main subunit of these receptors, in the striatum and the prefrontal cortex of rat brain were examined.

Material and Methods: For induction of morphine dependence, a dose of 10 mg/kg of morphine was administrated intraperitoneally for seven consecutive days. Control group received saline replace to morphine similarly. Tolerance to the morphine was examined by a hot plate test of analgesia, 1 day after the final repeated administrations. A semi-quantative RT-PCR was used for evaluating gene expression in different periods after withdrawal.

Results: The results showed that the level of mRNA of NR1 in the striatum on day 1 after the final repeated administrations of morphine was significantly increased, while in the prefrontal cortex was significantly decreased. on the other interval times of 3, 7, 14 and 21 days after the final repeated administrations of morphine, changes in the gene expression in the two areas compared with control group were not significant, and almost returned to the basal level.

 

Keywords: Morphine, NMDAR, Striatum, Prefrontal,cortex

Cloning and Sequencing of Mycobacterium tuberculosis ESAT-6 gene in pEGFP-N1 as an eukaryotic expres

Cloning and Sequencing of Mycobacterium tuberculosis ESAT-6 gene in pEGFP-N1 as an eukaryotic expressions vector

 

Mohammad Sadegh Koosha1*, Ali Mirjalili2,Majid Tebianian2, Garshasb Rigi Cheshmehali3,Gholamreza Ahmadian4

M.Sc in Biotechnology Engineering in Razi Vaccine and Serum Research Institute1

Assistant professor of Department of Biotechnology, Razi Vaccine and Serum Research Institute2

PhD student in Molecular Genetics in National Institute of Genetic Engineering and Biotechnology3

Assistant  professor of  Department of  Molecular Genetics in National Institute of Genetic Engineering and Biotechnology4

Introduction: Tuberculosis which has killed many people and caused many financial damages since many years ago is one of the major health problems. In spite of serious efforts to prevent disease, it is one of the important causes of death throughout world with two million death annually. One of the most important immunizing proteins of Mycobacterium tuberculosis is  ESAT–6 which is a 9.8KDa polypeptide and is  recognized  by T cells and stimulates  strong cell immunity responses  and  produces high amount  of  IFN–γ.

Objects and methods: This research was done in order to clone and sequence the ESAT–6 protein from Mycobacterium  tuberculosis  into a eukaryotic expression vector named pEGFP– N1.Characterizing of this gene by PCR cloning, restriction enzyme analysis and sequencing the gene can be the basis to produce a type of Stable Cell Line to be used for evaluation of ESAT-6 immune responses.

Results: Comparison of the nucleotide sequence of this gene and sequence of amino acid product of that with registered sequence in gene bank shows that this gene with isolated type (Rv3875) has high identity. This recombinant plasmid will be express on the CT-26 cell line which is a tumor cell from the BaIb/C mouse.

Key words: ESAT –6, pEGFP-N1, Cloning , Mycobacterium  tuberculosis.

Cloning and sequencig of the Infloanza A (H9N2) M2e - HSP70 fusion protein in prokaryotic expression

Cloning and sequencig of the Influenza A (H9N2) M2e - HSP70 fusion protein in prokaryotic expression vector in order to recombinant vaccine production

*Rigi cheshmehali Garshasb - Mashayekhpoor Saied-Aghaiepour Khosrow

            

Iran-Razi Vaccine and Serum Research Institute

*Corresponding author,s E. mail: garshasbbiotech@gmail.com

Abstract

      One of the concerns about influenza A vaccine based on M2e protein is their limited potency; hence, optimal approaches to enhance immunogenicity of M2e protein immunization remain to be established. It seems by linking this M2e-peptide to an appropriate carrier such as mycobacterium tuberculosis C-terminal 28-kDa domain of HSP70 (HSP70 359-610), we can render it very immunogenic.This study was designed to produce a novel influenza A virus recombinant fusion protein consisted of M2e , a potent immunogenic protein from influenza A virus, fused to C-terminal domain of mycobacterium tuberculosis HSP70, HSP70359-610 , as a carrier and adjuvant. We fused the genes of M2e and HSP70359-610 then inserted in pQE-60, prokaryotic expression vector PQE-60. Identification of the cloned gene was confirmed by PCR, restriction analysis and gene sequencing.Then cloned products were sent for sequencing to check the correct ORF and direct origin of the cloned gene. Comparison of the nocleotid sequence of this gene and sequence of amino acid product of that with registered sequences in genbank shows that this gene with total isolated types has much resemblance and after gene expression in prokaryotic system , it’s protein product can be a good candidate for recombinant vaccine against the Influenza.

 

Keywords: cloning-sequencing-Influenza A-M2e gene-HSP70fusion protein-PQE-60 vector-PCR.recombinant vaccine

cloning & the sequencing of HAO3 gene ( Hyalomma Anatolicum Anatolicum) in secretory shuttle vector

cloning & the sequencing of HAO3 gene ( Hyalomma Anatolicum Anatolicum) in secretory shuttle vector pPICZαA , Rigi cheshmehali  Garshasb , Aghayipor Khosrow , Mashayekhpour Saeid , Ebrahimi Seyed mahmoud , Razi Vaccine and Serum Research Institute

Abstract

     Hyalomma anatolicum anatulicum (Acari: Ixodidae) parasitize farm and wild animals in tropical and sub tropical region of the world. Ticks of the genus Hyalomma are well – khown vectors of viruses and avid parasits of man. One of the most important disease transmited by Hyalomma ticks is Crimean – Congo Hemorragic Fever (CCHF). H.anatolicum is one of the main vector ticks. In order to develop a broad – spectrum protection agianst different Hyalomma variants, some recent studies have been aimed at the HAO3 gene of Hyalomma anatolicum anatulicum.In this study, open reading frame of Hyalomma HAO3 gene was amplified by PCR using specific primers with restriction sites for XbaI and EcoRI restriction enzymes, cloned to pPICZαA expression vector in E.Coli DH5α. recimbinant vector was confirmed by PCR and restriction enzymes. Then cloned products were sent for sequencing to check the correct ORF and direct origin of the cloned HAO3 gene. Comparison of the nucleotid sequence of this gene and sequence of amino acid product of that with registered sequences in genbank shows that this gene with total isolated types HAO3, BM86, HA98 has much resemblance and after gene expression in eukaryotic yeast (pichia pastoris), it’s protein product can be a good candidate for recombinant vaccine against the Hyalomma.

 

Keywords: cloning-sequencing- PCR -HAO3gene- pPICZαA vector

 

Influenza vaccine production in plants

 

Review article

 Influenza vaccine production in plants

Rigi Cheshmehali Garshasb1 – Mousavi Seyyed Amir2

1- PhD student in Biology – Molecular Genetics in National Institute of Genetic Engineering and Biotechnology (NIGEB)- Iran

2-Director, Department of Plant Biotechnology & International Relations Coordinate of National Institute of Genetic Engineering and Biotechnology (NIGEB)- Iran

 

A b s t r a c t

              Plants have been identified as promising expression systems for commercial production of vaccine antigens. Transgenic plants, including edible plant parts are uggested as excellent alternatives for the production of vaccines and economic scale-up through cultivation. As an important case the spectre of an influenza pandemic has led to a revision of national and global pandemic preparedness plans and has stressed the need for more efficient influenza vaccines and manufacturing practices. This article reviews the current status of developments in the area of use of plants for the development of vaccine antigens against influenza.

 

Keywords: transgenic plants, vaccine antigens, influenza, pandemic.

 

 Introduction

       Infectious diseases account for more than 45% of total deaths in developing countries (9). Vaccination is the most effective means to prevent infectious diseases. More than 30 million children in the world are not immunized against treatable or preventable diseases (www.care.org/campaigns/childrenpowerty/facts.asp) because the currently used approaches to vaccine production are technologically complex and expensive. Specialized requirements of packaging, cold chain and mode of delivery add to the cost.

      Currently used mammalian cell line based vaccine manufacturing requires large investment and expertise. These factors limit their scale up and thus, global availability. Advances in molecular biology techniques during the 1980s, helped in the development of new strategies for the production of subunit vaccines. These comprised of proteins derived from pathogenic viruses, bacteria or parasites. Although mammals, their tissue sand cell lines are currently utilized for commercial production of vaccines, these systems are expensive and their scale up is not easy (9). Toxins, infectious agents and other noxious compounds get carried in animal cell based processes and are often difficult to remove. Such production systems are prone to microbial contamination which sometimes escapes detection even in purified vaccines. Expression of recombinant antigen proteins in E. coli is often not feasible because of lack of a variety of post translational modifications and folding requirements.

          Some of the mammalian-type post translational processing and modifications in protein happen in yeast and insect cell lines. However, immunologically significant differences in the pattern of post translational modifications limit their deployment in the expression of vaccine antigens. As a major alternative, plants are emerging as a promising system to express and manufacture a wide range of functionally active proteins of high value to health industry. Various plant biotechnological techniques, such as, modern breeding methods, clonal propagation , somatic hybridization, protoplast/cell suspension culture, hairy root culture and genetic transformation can play a vital role in establishing the use of plants as “surrogate production organisms”. One or more immunoprotective antigens of pathogens can be produced in plants by the expression of gene(s) encoding the protein(s). In recent years, plant-based novel production systems aimed at developing “edible” or “oral” vaccines have also been discussed(9). Compared to traditional vaccines, edible vaccines offer simplicity of use, lower cost, convenient storage, economic delivery and mucosal immune response. Successful development of vaccine antigens against human and animal pathogen(s) in plants requires selection of one or more immunoprotective antigens and designing of genes and promoters that would express the antigen(s) at a high level in target plant tissue. Genetic transformation methods are then utilized for introducing the gene in the target plant species (figure 1).

This review focuses on applications of plants for vaccine production against Influenza.

 Introducing the Influenza and importance of it’s plant- based vaccine

      Influenza is a highly contagious and acute respiratory disease with a high degree of morbidity and mortality. It is estimated that influenza is responsible for 36,000 deaths and more than 200,000 hospitalizations in the United States annually. Globally, influenza epidemics result in 3–5 million hospitalizations and 300,000–500,000 deaths each year (4). New epidemic strains of influenza A arise due to point mutations within two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA) (figure 2). These changes in HA and NA enable emerging virus strains to evade the host’s immune system and therefore necessitates the annual revision of vaccine to include the new viruses (3, 4). Influenza type A virus is classified into several subtypes which vary in two of their transmembrane proteins, hemagglutinin (HA)1 and neuraminidase (NA) and possess similar nucleoprotein and matrix protein (3,4). A constant antigenic reassortment in surface-located HA and NA, which are, respectively, responsible for binding of the virus to its cellular receptor and promoting release of the mature virus from infected cells (4), makes vaccine development a complicated process. There are 16 known HA subtypes and nine known NA subtypes of influenza A viruses and many different combinations are possible. Three subtypes, (H1N1, H1N2, and H3N2), commonly circulate among humans and all known subtypes of influenza A viruses can be found in birds (4). Influenza A subtype H5N1 virus, known as avian influenza or bird flu, is endemic mainly in birds and does not usually infect people. However, because of its high virulence, H5N1 may be transmitted from infected birds to humans and cause fatal disease.

 Figure 1 . Steps in the production of plant-derived vaccine antigens (9)

       The recent swine H1N1 influenza pandemic (pH1N1) revealed the limitations of the current influenza vaccine manufacturing technologies. Regardless of the origin, mechanism of emergence or precise genetic makeup of the ‘next’ pandemic strain, recent experience with pH1N1demonstrate clearly that the current, egg-based manufacturing system would not be able to respond quickly enough in the face of a highly pathogenic influenza virus adapted for rapid human-tohuman pread.

     A plant-based manufacturing technology that can produce vaccine doses within one month of

the sequencing of a pandemic strain should be describe.

 Figure 2 . Schematic representation of the structural characteristics of viral particles and plant-made VLPs (virus-like particles) .  A. Cross-section showing internal differences. Hemagglutinin (HA) (green) andneuraminidase (NA) (orange) are the two major viral proteins protruding outside of the viral envelope (pink) , HA being the main antigenic determinant of the virus.  B. Transmission electron microscopy images of influenza viruses and plant-made VLPs (3).

 Influenza vaccines produced in plants

       Evidence of influenza antigen production in plants was only recently disclosed, in 2004, through an international patent application (1). In this application, it is concluded that influenza HA accumulates in calluses of NT1 tobacco cells transformed to express the complete coding sequence of influenza H5 from strain A⁄ turkey ⁄ Wisconsin ⁄ 1968 (H5N9). When extracted without detergent, the apoplastic fluid of the transgenic cells exhibited hemagglutination activity, indicating that plant-produced H5 was secreted and active. Rabbits immunized with a crude preparation of extracellular fluid from transgenic NT-1 cells producing H5 from strain A⁄ turkey ⁄ Wisconsin ⁄ 1968 (H5N9) showed a strong hemagglutination inhibiting (HI) antibody response 6 weeks after immunization in the presence of Freund’s adjuvant. 

      Expression of the HA ectodomain (the segment of the protein spanning outside of the viral envelope) fused to a KDEL peptide, to enhance accumulation through retention in the endoplasmic reticulum, and a poly-histidine purification tag has also been utilized as a mean for facilitating the production of recombinant influenza antigens in plants. Examples include the HA ectodomains from a human seasonal influenza strain (A ⁄ Wyoming ⁄ 03 ⁄ 03 (H3N2) (6) and highly pathogenic avian strains A⁄ Indonesia ⁄ 5 ⁄ 05 (7), A⁄ Bar-headed Goose ⁄ Qinghai ⁄ 1A ⁄ 05 and A⁄ Anhui ⁄ 1 ⁄ 05 (8). In immunogenicity studies in mice, HA ectodomains have been shown to induce significant HI responses when administered in conjunction with Quil A. However, although such composition induced a strong immune response with doses as low as 1 lg (A ⁄ Anhui ⁄ 1 ⁄ 05 (H5N1)) (8), two doses were required to obtain an HI antibody response. A ferret study further showed that three doses of 45 lg adjuvanted with Quil A were required to confer protection against a lethal challenge with the homologous strain of H5 ectodomain (7). Together, the above mentioned immunogenicity studies, performed with HA fragments in fusion with a carrier protein or with other peptides, provided strong indications that plant-made influenza antigens can be produced by agroinfiltration and that these HA fragments induce hemagglutination inhibition antibody response in model animals. However, the high dosage and multiple injections required to induce a protective immune response in ferrets from immunization with the ectodomain of H5 (A ⁄ Indonesia ⁄ 5 ⁄ 05 (H5N1)) suggests that a higher order of antigen organization is required for optimal stimulation of a protective immune response. More recently, attempts at producing the entire H5 protein (from strain A⁄ Vietnam ⁄ 1203 ⁄ 04 (H5N1)) or its HA1 domain by transient or stable transformation of N. benthamiana were reported unsuccessful as they led to only detectable accumulation of the mature H5 or HA1 domain (10). Fragments of 34 or 27 kDa from the HA1 domain, or fusions of the HA1 domain with 26 kDa fragment from a human or a mouse heavy chain constant region were reported to accumulate at higher levels. In an immunogenicity study in mice, two doses of 10 lg of the 34 kDa fragment from the antigenic region of H5 from influenza A⁄ Vietnam ⁄ 1203 ⁄ 04 (H5N1), administered in the presence of alum-CpG as adjuvant, induced high H5 specific antibody titers but failed to induce significant HI antibody titers (10), again highlighting the need for a higher degree of organization of multivalent antigens to stimulate a protective immune response.

       A similar strategy was recently proposed in the international patent application WO2007⁄ 011904 for the presentation of influenza M2e universal epitope onto CPMV particles. Cowpea plants rubbed with RNA1 and chimeric RNA2 encoding a CP-M2e fusion produced chimeric CPMV particles comprising CP-M2e (5). Similarly, expression of a chimeric cucumber mosaic virus (CMV) capsid protein fused to the M2e epitope in N. benthamiana using a potato virus X (PVX) expression vector also led to the production of chimeric CP-M2e. However, assembly of the capsid proteins into chimeric viral particles was not demonstrated in this study (4). Capsid proteins of the PVX expression vector have also been used for the display of H-2Db-restricted epitope from the influenza NP from strain A⁄ PR ⁄ 8 ⁄ 34 (2). In this study, the epitope coding sequence was fused to the CP gene of the PVX vector, creating chimeric PVX particles displaying the NP epitope. An immunogenicity study in mice, designed to evaluate the cellbased immune response, showed that when administered in the presence of incomplete Freund’s adjuvant, 50 lg of chimeric viral particles displaying the NP epitope activated ASNENMETM-specific CD8+ IFN-c secreting cells. Although chimeric viral particles antigen presentation platforms benefit from the display of selected antigenic epitopes in a multivalent fashion, this strategy also bear some intrinsic drawbacks. Only a limited number of antigenic epitopes of small size (less than 25 amino acids) can be displayed using this system. Therefore, it is easier for rapidly evolving viruses like influenza to evade the immune response induced by such vaccines by replacing a few amino acids in the selected immunogenic region. Conformational epitopes may also not fold properly, thereby inducing the production of antibodies that will not recognize the cognate native epitope. Finally, the use of chimeric plant viral particles as presentation devices will require regulatory acceptance in themselves in addition to the regulatory hurdles faced by new manufacturing platform.

 Conclusions and future prospects

 With the world's population at over 6.4 billion (http://esa.un.org) , majority of the poor need affordable technological solutions to health. Protection from viral infections is currently the most difficult area to address through drug development. Progress in plant genetic engineering has opened novel opportunities to use plants as bioreactors for safe and cost effective production of   Influenza vaccine antigens.

      As is clear from several examples cited in this review, the production of recombinant proteins in plant systems has a great potential. Recent developments in this area have significantly increased its utility and enabled various groups to explore the possibility of producing vaccine antigens from a variety of plants, which can be directly or indirectly used to develop commercial processes. Transgenic plants that can produce biologically active proteins or subunit oral vaccines and antibodies have been developed, though the applications of these technologies are at least a decade away.

      The need to establish safety, efficacy and functional equivalence of the vaccine antigens should guide future development and research in plant based preventive and therapeutic technologies.

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2.                                  Lico, C., Mancini, C., Italiani, P., Betti, C., Boraschi, D., Benvenuto, E. and Baschieri, S. (2009) Plant-produced potato virus X chimeric particles displaying an influenza virus-derived peptide activate specific CD8+ T cells in mice. Vaccine, 27, 5069–5076.

 

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