A comprehensive review on staphylococcal protein A (SpA): Its production and applications

Authors

Garshasb Rigi, Samira Ghaedmohammadi, Gholamreza Ahmadian

Publication date

2019/3/14

Journal

Biotechnology and applied biochemistry

Description

The Staphylococcus aureus protein A (SpA) can be obtained through the culture of wild‐type S. aureus and also as a recombinant protein in safe bacterial hosts. Several methods have been used to purify SpA among which ion‐exchange chromatography, affinity chromatography, gel filtration, and per aqueous liquid chromatography (PALC) are common. SpA has a wide range of biochemical, biotechnological, and medical applications and is most commonly used in test methods such as immunoprecipitation, enzyme‐linked immunosorbent assay, and Western blotting. SpA has also been widely utilized in pharmaceutical applications to bind to immune complexes and serum immunoglobulins. SpA also directly binds to the B‐cells preventing initiation of infectious diseases as well as having a role in the development of various autoimmune diseases. This review considers different applications of SpA in …

Scholar articles

A comprehensive review on staphylococcal protein A (SpA): Its production and applications

G Rigi, S Ghaedmohammadi, G Ahmadian - Biotechnology and applied biochemistry, 2019